ANIONIC PHOSPHOLIPIDS CAN MEDIATE MEMBRANE INSERTION OF THE ANIONIC PART OF A BOUND PEPTIDE

被引：30

作者：

LEENHOUTS, JM

VANDENWIJNGAARD, PWJ

DEKROON, AIPM

DEKRUIJFF, B

机构：

[1] Department of Biochemistry of Membranes (CBLE), Institute of Biomembranes, Utrecht University, 3584 CH Utrecht

来源：

FEBS LETTERS | 1995年 / 370卷 / 03期

关键词：

ANIONIC PHOSPHOLIPID; PEPTIDE INSERTION; FLUORESCENCE SPECTROSCOPY; SURFACE PH;

D O I：

10.1016/0014-5793(95)00823-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of anionic lipids on the membrane insertion of a carboxyl group on a specially designed palmitoylated peptide was studied, using tryptophan fluorescence. It is demonstrated that the negatively charged membrane surface of mixed phosphatidylchoIine/phosphatidylglycerol small unilamellar vesicles enhances the protonation of the C-terminal carboxyl group, and the subsequent insertion of that part of the peptide.

引用

页码：189 / 192

页数：4

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