EFFECT OF ALCOHOLS ON THE ASSOCIATION OF PHOTOSYNTHETIC FRUCTOSE-1,6-BISPHSPHATASE TO THYLAKOID MEMBRANES

被引：5

作者：

ANDRES, AR ^{[1
]}

LAZARO, JJ ^{[1
]}

CHUECA, A ^{[1
]}

HERMOSO, R ^{[1
]}

GORGE, JL ^{[1
]}

机构：

[1] CSIC,ESTAC EXPTL ZAIDIN,PROF ALBAREDA 1,E-18008 GRANADA,SPAIN

来源：

PHYSIOLOGIA PLANTARUM | 1990年 / 78卷 / 03期

关键词：

Alcohols; binding; chloroplast membranes; fructose‐1,6‐bisphosphatase; pea; Pisum sativum;

D O I：

10.1111/j.1399-3054.1990.tb09056.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Aliphatic alcohols have a positive effect on the assoociation of pea (Pisum sativum L. cv. Lincoln) chloroplast fructose‐ 1,6‐bisphosphatase (FBPase; EC 3.1.3.11) with thylakoid membranes. The alcohol concentration needed to obtain a fixed percentage of enzyme association decreased with increased length of the aliphatic chain of the alcohol; maximum binding was obtained when the lysis medium contained, in molar fractions (or v/v percentages): 48×10‐4(T4 (2.4%), 26×10‐3 (10%), 40×10‐3 (15%), 76×10‐3 (21%), and 13×10‐2 (24%), of 1‐butanol, 1‐propanol, 2‐propanol, ethanol, and methanol, respectively. A good correlation of binding with the octanol/water partition coefficient was observed. Since this coefficient constitutes a measure of hydrophobicity, we suggest that the binding of FBPase to the membranes occurs via hydrophobic clusters of both components. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：409 / 413

页数：5

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