IDENTIFICATION OF A G1-S-PHASE-REGULATED REGION IN THE HUMAN THYMIDINE KINASE GENE PROMOTER

被引：52

作者：

ROEHL, HH ^{[1
]}

CONRAD, SE ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV, DEPT MICROBIOL & PUBL HLTH, E LANSING, MI 48824 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 07期

关键词：

D O I：

10.1128/MCB.10.7.3834

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a regulatory region in the human thymidine kinase gene promoter. A set of promoter deletion mutants was constructed, linked to the bacterial neomycin resistance gene, and stably transfected into Rat3 cells. It was shown that the region between 135 and 67 base pairs upstream of the cap site is required for conveying G1-S-phase regulation to the linked neo gene. In addition, primer extension assays demonstrated that the same transcriptional start sites were used in G1- and S-phase cells and in the various deletion mutants tested.

引用

页码：3834 / 3837

页数：4

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