ISOLATION AND CHARACTERIZATION OF BOVINE GINGIVAL PROTEOGLYCANS VERSICAN AND DECORIN

被引：20

作者：

BRATT, P

ANDERSON, MM

MANSSONRAHEMTULLA, B

STEVENS, JW

ZHOU, C

RAHEMTULLA, F

机构：

[1] UNIV ALABAMA,SCH DENT,DEPT COMMUNITY & PUBL HLTH DENT,UNIV STN,BIRMINGHAM,AL 35294

[2] UMEA UNIV,DEPT CARIOL,S-90187 UMEA,SWEDEN

[3] UNIV ALABAMA,DEPT MED,BIRMINGHAM,AL 35294

[4] UNIV ALABAMA,SCH DENT,DEPT ORAL BIOL,BIRMINGHAM,AL 35294

[5] UNIV ALABAMA,VET ADM MED CTR,BIRMINGHAM,AL 35294

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1992年 / 24卷 / 10期

关键词：

D O I：

10.1016/0020-711X(92)90173-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. We have isolated, chemically and immunologically characterized versican and decorin from bovine gingiva. 2. Versican was of large molecular weight and the molecular size of the core protein was estimated to be greater than 200 kDa. 3. The glycosaminoglycan chains were susceptible to chondroitinase ABC and N-linked oligosaccharides were present on the protein core of the molecule. 4. Immunological studies provided evidence that a hyaluronic acid binding region was present in the core protein of versican. 5. The overall structure was similar to that of versican isolated from bovine sclera. 6. Decorin had a molecular weight of 102 kDa and its glycosaminoglycan chain was completely digested by specific glycosidases. 7. The partially deglycosylated core protein had a molecular weight of 55 kDa and N-linked oligosaccharides were present on the molecule.

引用

页码：1573 / 1583

页数：11

共 53 条

[1] PROTEOGLYCANS OF THE PERIODONTIUM - STRUCTURE, ROLE AND FUNCTION [J].