Isolation and sequence determination of the cDNA encoding DNA polymerase delta from Drosophila melanogaster

被引：11

作者：

Chiang, CS ^{[1
]}

Lehman, IR ^{[1
]}

机构：

[1] STANFORD UNIV,BECKMAN CTR,DEPT BIOCHEM,STANFORD,CA 94305

来源：

GENE | 1995年 / 166卷 / 02期

关键词：

DNA replication; PCNA; cloning; PCR; antibody; sequence conservation;

D O I：

10.1016/0378-1119(95)00567-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The cDNA encoding the catalytic subunit of Drosophila melanogaster (Dm) DNA polymerase delta (Pol delta) was isolated by a combination of PCR amplification and cDNA library screening. The cDNA is 3457 nucleotides in length and contains an open reading frame (ORF) that encodes a protein of 1092 amino acids (124 799 Da). The ORF contains the sequence that was determined for a peptide from the purified catalytic subunit of Dm Pol delta. Polyclonal antibodies raised against Dm Pol delta specifically recognize a protein of the expected size when the cDNA is expressed in either Escherichia coli or insect cells. Comparison of the deduced aa sequence with other Pol delta sequences demonstrates that Pol delta is one of the most highly conserved of the DNA polymerases.

引用

页码：237 / 242

页数：6

共 36 条

[1]

ABUSSEKHRA A, 1995, CELL, V80, P859

[2] THE YEAST ANALOG OF MAMMALIAN CYCLIN PROLIFERATING-CELL NUCLEAR ANTIGEN INTERACTS WITH MAMMALIAN DNA POLYMERASE-DELTA [J].

BAUER, GA ;

BURGERS, PMJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7506-7510

[3] A CONSERVED 3'-]5' EXONUCLEASE ACTIVE-SITE IN PROKARYOTIC AND EUKARYOTIC DNA-POLYMERASES [J].

BERNAD, A ;

BLANCO, L ;

LAZARO, JM ;

MARTIN, G ;

SALAS, M .

CELL, 1989, 59 (01) :219-228

[4] THE HIGHLY CONSERVED AMINO-ACID-SEQUENCE MOTIF TYR-GLY-ASP-THR-ASP-SER IN ALPHA-LIKE DNA-POLYMERASES IS REQUIRED BY PHAGE-PHI-29 DNA-POLYMERASE FOR PROTEIN-PRIMED INITIATION AND POLYMERIZATION [J].

BERNAD, A ;

LAZARO, JM ;

SALAS, M ;

BLANCO, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (12) :4610-4614

[5] STRUCTURE AND FUNCTION OF THE SACCHAROMYCES-CEREVISIAE CDC2 GENE ENCODING THE LARGE SUBUNIT OF DNA POLYMERASE-III [J].

BOULET, A ;

SIMON, M ;

FAYE, G ;

BAUER, GA ;

BURGERS, PMJ .

EMBO JOURNAL, 1989, 8 (06) :1849-1854

[6]

BROWN WC, 1993, J BIOL CHEM, V268, P21706

[7]

BUDD ME, 1995, MOL CELL BIOL, V15, P2173

[8] COMPARISON OF THE CONSENSUS SEQUENCE FLANKING TRANSLATIONAL START SITES IN DROSOPHILA AND VERTEBRATES [J].

CAVENER, DR .

NUCLEIC ACIDS RESEARCH, 1987, 15 (04) :1353-1361

[9] DNA POLYMERASE-DELTA FROM EMBRYOS OF DROSOPHILA-MELANOGASTER [J].

CHIANG, CS ;

MITSIS, PG ;

LEHMAN, IR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (19) :9105-9109

[10] PRIMARY STRUCTURE OF THE CATALYTIC SUBUNIT OF HUMAN DNA POLYMERASE-DELTA AND CHROMOSOMAL LOCATION OF THE GENE [J].

CHUNG, DW ;

ZHANG, J ;

TAN, CK ;

DAVIE, EW ;

SO, AG ;

DOWNEY, KM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (24) :11197-11201

← 1 2 3 4 →