CONSTRUCTION OF LINEAR GLCNAC-BETA-1-6GAL-BETA-1-OR TYPE OLIGOSACCHARIDES BY PARTIAL CLEAVAGE OF GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL-BETA-1-OR SEQUENCES WITH JACK BEAN BETA-N-ACETYLHEXOSAMINIDASE

被引：13

作者：

RENKONEN, O ^{[1
]}

NIEMELA, R ^{[1
]}

LEPPANEN, A ^{[1
]}

MAAHEIMO, H ^{[1
]}

SEPPO, A ^{[1
]}

PENTTILA, L ^{[1
]}

VILKMAN, A ^{[1
]}

机构：

[1] UNIV HELSINKI,DEPT BIOCHEM,SF-00380 HELSINKI,FINLAND

来源：

GLYCOCONJUGATE JOURNAL | 1991年 / 8卷 / 04期

关键词：

OLIGO-N-ACETYLLACTOSAMINOGLYCANS; ENZYMATIC SYNTHESIS; INVITRO; GLCNAC-BETA-1-6GAL-BETA-1-4GLCNAC/GLC; GLCNA-BETA-1-3(GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC/GLC; GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL; KINETICS OF BETA-N-ACETYLHEXOSAMINIDASE CLEAVAGE; INTRAMOLECULAR INTERACTIONS;

D O I：

10.1007/BF00731350

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Radiolabelled GlcNAc-beta-1-3(GlcNAc-beta-1-6)Gal (1), GlcNAc-beta-1-3(GlcNAc-beta-1-6)Gal-beta-1-OCH3 (4), GlcNAc-beta-1-3(GlcNAc-beta-1-6)Gal-beta-1-4Glc (7), and GlcNAc-beta-1-3(GlcNAc-beta-1-6)Gal-beta-1-4GlcNAc (10) were cleaved partially with jack bean beta-N-acetylhexosaminidase (EC 3.2.1.30), and the digests were analysed chromatographically. All four oligosaccharides were hydrolysed faster at the 91-6) branch, than at the (1-3) branch, but a high branch specificity was observed only with the glycan 4. The saccharides 1 and 7 resembled each other in the kinetics of the enzyme-catalysed release of their two non-reducing N-acetylglucosamine units, but the glycan 10 was rather different. The partial digestions made it possible to obtain radiolabelled GlcNAc-beta-1-6Gal, GlcNAc-beta-1-6Gal-beta-1-OCH3, GlcNAc-beta-1-6Gal-beta-1-4Glc, and, in particular, GlcNAc-beta-1-6Gal-beta-1-4GlcNAc.

引用

页码：368 / 375

页数：8

共 13 条

[1]

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