DETECTION OF DYE-LINKED D-MANNITOL DEHYDROGENASE-ACTIVITY IN ACETOBACTER-XYLINUM KU-1

被引：4

作者：

OIKAWA, T

AMEYAMA, M

机构：

[1] Department of Biotechnology, Kansai University, Osaka, 564, Suita

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 09期

关键词：

D O I：

10.1271/bbb.57.1580

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We detected dye-linked D-mannitol dehydrogenase activity in the crude extract of Acetobacter xylinum KU-1. The enzyme activity was specific for D-mannitol, and not pyridine nucleotide (NAD+, NADP+)-dependent. The optimal pH was found to be 5.0, while the optimal temperature was at 50-degrees-C. The enzyme activity was inhibited by p-quinone noncompetitively.

引用

收藏

页码：1580 / 1581

页数：2

相关论文

共 17 条

[1] CRYSTALLIZATION OF MEMBRANE-BOUND ALCOHOL-DEHYDROGENASE OF ACETIC-ACID BACTERIA [J].

ADACHI, O ;

SHINAGAWA, E ;

MATSUSHITA, K ;

AMEYAMA, M .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (11) :2859-2863

[2] D-GLUCOSE DEHYDROGENASE OF GLUCONOBACTER-SUBOXYDANS - SOLUBILIZATION, PURIFICATION AND CHARACTERIZATION [J].

AMEYAMA, M ;

SHINAGAWA, E ;

MATSUSHITA, K ;

ADACHI, O .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1981, 45 (04) :851-861

[3] SOLUBILIZATION, PURIFICATION AND PROPERTIES OF MEMBRANE-BOUND GLYCEROL DEHYDROGENASE FROM GLUCONOBACTER-INDUSTRIUS [J].

AMEYAMA, M ;

SHINAGAWA, E ;

MATSUSHITA, K ;

ADACHI, O .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1985, 49 (04) :1001-1010

[4] PURIFICATION AND PROPERTIES OF A POLYOL DEHYDROGENASE FROM CEPHALOSPORIUM-CHRYSOGENUS [J].

BIRKEN, S ;

PISANO, MA .

JOURNAL OF BACTERIOLOGY, 1976, 125 (01) :225-232

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6]

CHAKRAVORTY M, 1962, J BIOL CHEM, V237, P1014

[7] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].

DAVIS, BJ .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) :404-&

[8]

EDMUNDOWICZ JM, 1963, J BIOL CHEM, V238, P3539

[9]

HORWITZ SB, 1964, J BIOL CHEM, V239, P830

[10]

KERSTERS K, 1966, METHOD ENZYMOL, V9, P170