EFFECTS OF INTERFERON-GAMMA ON CELL-DIFFERENTIATION AND CYTOKINE PRODUCTION OF A HUMAN MONOBLAST CELL-LINE, U937

被引：14

作者：

ISHIZUKA, T

HIRATA, I

ADACHI, M

KURIMOTO, F

HISADA, T

DOBASHI, K

MORI, M

机构：

[1] JAPAN IMMUNORES LABS CO LTD,TAKASAKI,GUMMA 37012,JAPAN

[2] MITSUBISHI KAGAKU BIOCLIN LABS INC,ITABASHI KU,TOKYO 174,JAPAN

来源：

INFLAMMATION | 1995年 / 19卷 / 06期

关键词：

D O I：

10.1007/BF01534567

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The U937 cell, a human monoblast cell line, has been used as a model to study the function of human monocytes. We investigated the effects of interferon-gamma (IFN-gamma) on superoxide anion (O-2(-)) production, cell surface antigens, and cytokine production of U937 cells. IFN-gamma treatment enhanced O-2(-) production of fMLP or PMA-stimulated U937 cells. IFN-gamma increased the ratio of CD23 and CD11b positive cells. The fluorescence intensity of CD14 and CD25 was enhanced by IFN-gamma treatment. U937 cells produced IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha by lipopolysaccharide (LPS) stimulation. IFN-gamma treatment enhanced TNF-or production, but decreased IL-6 production. These results suggest that IFN-gamma differentiates U937 cells to monocytelike cells and it regulates the production systems of IL-6 and TNF-alpha separately in U937 cells.

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收藏

页码：627 / 636

页数：10

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