MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A NOVEL AMILORIDE-SENSITIVE NA+ CHANNEL

被引：265

作者：

WALDMANN, R ^{[1
]}

CHAMPIGNY, G ^{[1
]}

BASSILANA, F ^{[1
]}

VOILLEY, N ^{[1
]}

LAZDUNSKI, M ^{[1
]}

机构：

[1] INST PHARMACOL MOLEC & CELLULAIRE,F-06560 VALBONNE,FRANCE

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 46期

关键词：

D O I：

10.1074/jbc.270.46.27411

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a cDNA for a novel human amiloride-sensitive Na+ channel isoform (called delta) which is expressed mainly in brain, pancreas, testis, and ovary. When expressed in Xenopus oocytes, it generates an amiloride-sensitive Na+ channel with biophysical and pharmacological properties distinct from those of the epithelial Na+ channel, a multimeric assembly of alpha, beta, and gamma subunits. The Na+ current produced by the new delta isoform is increased by two orders of magnitude after coexpression of the beta and gamma subunit of the epithelial Na+ channel showing that delta can associate with other subunits and is part of a novel multisubunit ion channel.

引用

页码：27411 / 27414

页数：4

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