A MICROTITER CELL-CULTURE ASSAY FOR THE DETERMINATION OF ANTI-HUMAN IMMUNODEFICIENCY VIRUS NEUTRALIZING ANTIBODY-ACTIVITY

被引：56

作者：

ROBERTSON, GA ^{[1
]}

KOSTEK, BM ^{[1
]}

SCHLEIF, WA ^{[1
]}

LEWIS, JA ^{[1
]}

EMINI, EA ^{[1
]}

机构：

[1] MERCK SHARP & DOHME RES LABS, DEPT VIRUS & CELL BIOL, W POINT, PA 19486 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1988年 / 20卷 / 03期

关键词：

D O I：

10.1016/0166-0934(88)90123-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microtiter cell-culture assay is described for measuring neutralizing antibody activity directed against the human immunodeficiency virus(HIV). The assay relies upon inhibition of HIV-mediated cell killing of infected MT-4 lymphoid cells. The assay exhibits comparable sensitivity to two other methods used for such measurements, is relatively rapid, may be adapted for screening large numbers of samples and involves minimal handling of infectious virus.

引用

页码：195 / 202

页数：8

共 18 条

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