FATTY-ACID CHAIN IS A CRITICAL EPITOPE FOR ANTIPHOSPHOLIPID ANTIBODY

被引：28

作者：

LEVY, RA ^{[1
]}

GHARAVI, AE ^{[1
]}

SAMMARITANO, LR ^{[1
]}

HABINA, L ^{[1
]}

LOCKSHIN, MD ^{[1
]}

机构：

[1] CORNELL UNIV,MED CTR,COLL MED,HOSP SPECIAL SURG,DIV RHEUMAT DIS,NEW YORK,NY 10021

来源：

JOURNAL OF CLINICAL IMMUNOLOGY | 1990年 / 10卷 / 03期

关键词：

Antiphospholipid antibody; cardiolipin; fatty acid; membrane;

D O I：

10.1007/BF00917913

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To explore the role of phospholipid fatty acids in binding of antiphospholipid antibody (aPL) in ELISA, we tested aPL binding to phospholipids containing fatty acids of varying chain length and degree of saturation using direct ELISA and inhibition methods. Polyclonal IgG and IgM human aPL's bind to C18:1 phosphatidylglycerol (PG) better than to C18:0 PG or C18:2 PG. Binding is greater to C18 than to C14:0 or C16:0 PGs; aPL's do not bind to C12:0 PG. aPL binding is not inhibited by C18:1 diacylglycerol, glycerol-3-phosphate, myoinositol, or myoinositol phosphate. The fatty acid chains are critical determinants for antigen recognition and, by projection, biological activity of aPL. © 1990 Plenum Publishing Corporation.

引用

页码：141 / 145

页数：5

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