DETECTION OF A TRANSCRIPTIONAL BLOCK IN THE 1ST INTRON OF THE HUMAN C-MYB GENE

被引：11

作者：

CASTELLANO, M ^{[1
]}

GOLAY, J ^{[1
]}

MANTOVANI, A ^{[1
]}

INTRONA, M ^{[1
]}

机构：

[1] MARIO NEGRI INST PHARMACOL RES,VIA ERITREA 62,I-20157 MILAN,ITALY

来源：

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH | 1992年 / 22卷 / 03期

关键词：

C-MYB PROTOONCOGENE; TRANSCRIPTIONAL BLOCK;

D O I：

10.1007/BF02591416

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The levels of expression of the murine c-myb gene, like those of several other proto-oncogenes, can be controlled by a block of transcriptional elongation within the first intron of the gene. We have performed run-off experiments with double- and single-stranded probes on the myelomonocytic cell line U937, and show that this mechanism of transcriptional arrest is true also for the human c-myb gene and takes place within the first intron. Furthermore, we have sequenced the entire first intron of the human c-myb gene, and discuss the sequence structure in relation to its putative ability to arrest RNA polymerase II and its high degree of homology with the equivalent murine intron.

引用

页码：159 / 164

页数：6

共 20 条

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