EFFECT OF TRANSFORMING GROWTH-FACTOR-BETA ON FIBROBLASTS IN 3-DIMENSIONAL LATTICE CULTURES

被引：19

作者：

COUSTRY, F

GILLERY, P

MAQUART, FX

BOREL, JP

机构：

[1] Laboratory of Biochemistry, CNRS URA 610, University of Reims, 51095 Reims Cedex

来源：

FEBS LETTERS | 1990年 / 262卷 / 02期

关键词：

Collagen lattice; Collagen synthesis; Fibrin lattice; Fibroblast culture; Transforming growth factor β;

D O I：

10.1016/0014-5793(90)80223-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human skin fibroblasts were cultivated in three-dimensional fibrin or collagen lattices, under retracting or non-retracting conditions, and the influence of transforming growth factor β (TGFβ) was tested. TGFβ stimulated the synthesis of non-collagen protein and of collagen in all the systems. However, only in non-retracting fibrin lattices did it restore a level of protein synthesis comparable to that found in monolayers. The effects of TGFβ greatly depended on the type of substratum and on the presence or absence of retraction. © 1990.

引用

页码：339 / 341

页数：3

共 16 条

[1]

ASSOIAN RK, 1983, J BIOL CHEM, V258, P7155

[2]

BASSOLS A, 1988, J BIOL CHEM, V263, P3039

[3] PRODUCTION OF A TISSUE-LIKE STRUCTURE BY CONTRACTION OF COLLAGEN LATTICES BY HUMAN-FIBROBLASTS OF DIFFERENT PROLIFERATIVE POTENTIAL INVITRO [J].