DNA TRANSFECTION OF MOUSE LYMPHOID-CELLS BY THE COMBINATION OF DEAE-DEXTRAN-MEDIATED DNA UPTAKE AND OSMOTIC SHOCK PROCEDURE

被引：69

作者：

TAKAI, T ^{[1
]}

OHMORI, H ^{[1
]}

机构：

[1] OKAYAMA UNIV,FAC ENGN,DEPT BIOTECHNOL,TSUSHIMA NAKA 3-1-1,OKAYAMA 700,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1048卷 / 01期

关键词：

(Mouse lymphoid cell); DEAE-dextran; DNA transfection; Osmotic shock;

D O I：

10.1016/0167-4781(90)90029-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several mouse lymphoid cell lines were efficiently transfected with plasmid DNA by a novel method combining DEAE-dextran-mediated DNA uptake and osmotic shock procedure. The cells were first incubated with DNA-DEAE-dextran complex, treated with hypertonic Tris-HCl buffer containing 0.5 M sucrose and 10% poly(ethylene glycol), and then exposed to hypotonic RPMI 1640 medium. This transfection protocol exhibited maximal frequencies of 0.3% and 3·10-5 for transient gene expression and stable transformation in P3-NSI/1-Ag4-1 cells, respectively. © 1990.

引用

页码：105 / 109

页数：5

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