PURIFICATION OF ALPHA-L-FUCOSIDASE BY C-GLYCOSYLIC AFFINITY-CHROMATOGRAPHY, AND THE ENZYMATIC-SYNTHESIS OF ALPHA-L-FUCOSYL DISACCHARIDES

An α-l-fucosidase from porcine liver was purified using the new C-glycosylic affinity adsorbent, Sepharose-ε-aminocaproyl-3-(α-l-fucopyranosyl)propylamine. The C-fucosylic linkage was synthesized by a radical reaction of 2,3,4-tri-O-acetyl-α-l-fucopyranosyl bromide with acrylonitrile. In transglycosylation reactions with p-nitrophenyl α-l-fucopyranoside or α-l-fucopyranosyl fluoride as donor and methyl β-d-galactopyranoside as acceptor, the enzyme mediated the formation of (1→2)- and (1→6)-linked-α-l-fucosyl derivatives (6.5 and 10.0%, respectively). © 1990.