COMPLETE H-1 AND C-13 ASSIGNMENT OF LYS AND LEU SIDE-CHAINS OF STAPHYLOCOCCAL NUCLEASE USING HCCH-COSY AND HCCH-TOCSY-3D NMR-SPECTROSCOPY

被引：7

作者：

BALDISSERI, DM ^{[1
]}

PELTON, JG ^{[1
]}

SPARKS, SW ^{[1
]}

TORCHIA, DA ^{[1
]}

机构：

[1] NIDR,BONE RES BRANCH,BETHESDA,MD 20892

来源：

FEBS LETTERS | 1991年 / 281卷 / 1-2期

关键词：

STAPHYLOCOCCAL NUCLEASE; SIDE-CHAIN STRUCTURE; ASSIGNMENT; 3D NMR;

D O I：

10.1016/0014-5793(91)80352-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complete proton and carbon sidechain assignments are reported for 22 lysine and 11 leucine residues in staphylococcal nuclease, an enzyme with 149 residues. These assignments are readily obtained in a direct manner from the correlations observed in the 3D'HCCH-COSY and HCCH-TOCSY spectra and the known protein backbone assignments. These assignments open the way to detailed studies of the sidechain structure and dynamics at the active site, in the hydrophobic core and on the surface of the protein.

引用

页码：33 / 38

页数：6

共 20 条

[1] H-1-H-1 CORRELATION VIA ISOTROPIC MIXING OF C-13 MAGNETIZATION, A NEW 3-DIMENSIONAL APPROACH FOR ASSIGNING H-1 AND C-13 SPECTRA OF C-13-ENRICHED PROTEINS
BAX, A
CLORE, GM
GRONENBORN, AM
[J]. JOURNAL OF MAGNETIC RESONANCE, 1990, 88 (02): : 425 - 431
[2] PRACTICAL ASPECTS OF PROTON CARBON CARBON PROTON 3-DIMENSIONAL CORRELATION SPECTROSCOPY OF C-13-LABELED PROTEINS
BAX, A
CLORE, GM
DRISCOLL, PC
GRONENBORN, AM
IKURA, M
KAY, LE
[J]. JOURNAL OF MAGNETIC RESONANCE, 1990, 87 (03): : 620 - 627
[3] ASSIGNMENT OF THE SIDE-CHAIN H-1 AND C-13 RESONANCES OF INTERLEUKIN-1-BETA USING DOUBLE-RESONANCE AND TRIPLE-RESONANCE HETERONUCLEAR 3-DIMENSIONAL NMR-SPECTROSCOPY
CLORE, GM
BAX, A
DRISCOLL, PC
WINGFIELD, PT
GRONENBORN, AM
[J]. BIOCHEMISTRY, 1990, 29 (35) : 8172 - 8184
[4] COMPLETE RESONANCE ASSIGNMENT FOR THE POLYPEPTIDE BACKBONE OF INTERLEUKIN-1-BETA USING 3-DIMENSIONAL HETERONUCLEAR NMR-SPECTROSCOPY
DRISCOLL, PC
CLORE, GM
MARION, D
WINGFIELD, PT
GRONENBORN, AM
[J]. BIOCHEMISTRY, 1990, 29 (14) : 3542 - 3556
[5] 2D AND 3D NMR-SPECTROSCOPY EMPLOYING C-13-C-13 MAGNETIZATION TRANSFER BY ISOTROPIC MIXING - SPIN SYSTEM-IDENTIFICATION IN LARGE PROTEINS
FESIK, SW
EATON, HL
OLEJNICZAK, ET
ZUIDERWEG, ERP
MCINTOSH, LP
DAHLQUIST, FW
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (02) : 886 - 888
[6] A NOVEL-APPROACH FOR SEQUENTIAL ASSIGNMENT OF H-1, C-13, AND N-15 SPECTRA OF LARGER PROTEINS - HETERONUCLEAR TRIPLE-RESONANCE 3-DIMENSIONAL NMR-SPECTROSCOPY - APPLICATION TO CALMODULIN
IKURA, M
KAY, LE
BAX, A
[J]. BIOCHEMISTRY, 1990, 29 (19) : 4659 - 4667
[7] 3-DIMENSIONAL NOESY-HMQC SPECTROSCOPY OF A C-13-LABELED PROTEIN
IKURA, M
KAY, LE
TSCHUDIN, R
BAX, A
[J]. JOURNAL OF MAGNETIC RESONANCE, 1990, 86 (01): : 204 - 209
[8] PROTON PROTON CORRELATION VIA CARBON CARBON COUPLINGS - A 3-DIMENSIONAL NMR APPROACH FOR THE ASSIGNMENT OF ALIPHATIC RESONANCES IN PROTEINS LABELED WITH C-13
KAY, LE
IKURA, M
BAX, A
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (02) : 888 - 889
[9] THE CRYSTAL-STRUCTURE OF THE TERNARY COMPLEX OF STAPHYLOCOCCAL NUCLEASE, CA-2+, AND THE INHIBITOR PDTP, REFINED AT 1.65-A
LOLL, PJ
LATTMAN, EE
[J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1989, 5 (03): : 183 - 201
[10] 3-DIMENSIONAL HETERONUCLEAR NMR OF N-15-LABELED PROTEINS
MARION, D
KAY, LE
SPARKS, SW
TORCHIA, DA
BAX, A
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (04) : 1515 - 1517

← 1 2 →