A SIMPLE PROCEDURE FOR THE PREPARATION OF LIPOPOLYSACCHARIDE FOR THE PRODUCTION OF ANTISERA

被引：10

作者：

CHART, H

ROWE, B

机构：

[1] Division of Enteric Pathogens, Central Public Health Laboratory, London, NW9 5HT

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1992年 / 14卷 / 06期

关键词：

D O I：

10.1111/j.1472-765X.1992.tb00701.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipopolysaccharide (LPS) was isolated from a strain of Escherichia coli O157 by two different methods and used to prepare antisera in rabbits. LPS prepared by the proteolytic digestion of whole cells was identical to LPS prepared with hot-phenol, as shown by SDS-PAGE and silver staining. Antisera from rabbits receiving these LPS preparations contained high titred antibodies (> 10(5)) of the IgM class to E. coli O157 LPS.

引用

页码：263 / 265

页数：3

共 5 条

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