CLASTOGENIC AND ANEUPLOIDIZING EFFECTS OF ANTIBLASTIC BUSULFAN REVEALED BY KINETOCHORE IMMUNOFLUORESCENCE IN CHO CELLS

被引：4

作者：

BARBATA, G ^{[1
]}

GIGLIO, MC ^{[1
]}

GRANATA, G ^{[1
]}

ANZALONE, A ^{[1
]}

CARBONE, P ^{[1
]}

机构：

[1] UNIV PALERMO,DIPARTIMENTO BIOL CELLULARE & SVILUPPO A MONROY,SEZ GENET,I-90123 PALERMO,ITALY

来源：

MUTATION RESEARCH | 1991年 / 263卷 / 04期

关键词：

BUSULFAN; MICRONUCLEUS ASSAY; KINETOCHORE IMMUNOFLUORESCENCE; ANEUPLOIDY; CHO CELLS;

D O I：

10.1016/0165-7992(91)90007-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We utilized, in CHO cells, the cytoplasm preservation technique to evaluate the micronucleus frequency at different busulphan concentrations, and the indirect immunofluorescence technique, using sera obtained from patients with scleroderma (CREST variant), to analyze if busulphan-induced micronuclei have kinetochores. Results show that this alkylating agent is capable of causing a significant increase of micronuclei in vitro, a great part (40%) of them having CREST-positive kinetochores. These findings confirm the clastogenic effect of busulphan and reveal a considerable capability of this agent to induce aneuploidy. These results are examined taking into account the high incidence of secondary neoplasias induced by chemotherapy with alkylating agents utilized against primary neoplasias.

引用

页码：237 / 242

页数：6

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