APPLICATION OF TOTALLY AUTOMATED ONLINE SAMPLE CLEAN UP FOR PROSTANOID EXTRACTION AND HPLC SEPARATION

被引：5

作者：

HOTTER, G ^{[1
]}

RAMIS, I ^{[1
]}

BIOQUE, G ^{[1
]}

SARMIENTO, C ^{[1
]}

FERNANDEZ, JM ^{[1
]}

ROSELLOCATAFAU, J ^{[1
]}

GELPI, E ^{[1
]}

机构：

[1] MICROBEAM,BARCELONA,SPAIN

来源：

CHROMATOGRAPHIA | 1993年 / 36卷

关键词：

COLUMN LIQUID CHROMATOGRAPHY; PROSTAGLANDINS; C-18 SOLID PHASE EXTRACTION; RADIOIMMUNOANALYSIS;

D O I：

10.1007/BF02263832

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study has been the evaluation of an automated system for on-line sample preparation using solid phase extraction and HPLC purification for the measurement of prostanoids in urine. We have established the optimum precolumn and column conditions for this analysis. The manual extraction - HPLC procedure furnishes lower recoveries and higher coefficients of variation than those obtained by the automated on-line procedure. The automated system has been applied to prostanoid analysis of human urine samples from subjects exposed to lead.

引用

页码：33 / 38

页数：6

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