DETECTION OF VIRULENCE FACTORS IN CULTURABLE ESCHERICHIA-COLI ISOLATES FROM WATER SAMPLES BY DNA PROBES AND RECOVERY OF TOXIN-BEARING STRAINS IN MINIMAL ORTHO-NITROPHENOL-BETA-D-GALACTOPYRANOSIDE-4-METHYLUMBELLIFERYL-BETA-D-GLUCURONIDE MEDIA

被引：21

作者：

MARTINS, MT

RIVERA, IG

CLARK, DL

OLSON, BH

机构：

[1] UNIV CALIF IRVINE,SCH SOCIAL ECOL,IRVINE,CA 92717

[2] UNIV SAO PAULO,DEPT MICROBIOL,BR-05508 SAO PAULO,BRAZIL

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 09期

关键词：

D O I：

10.1128/AEM.58.9.3095-3100.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A total of 449 Escherichia coli isolates in treated and raw water sources were submitted to DNA-DNA hybridization using seven different DNA probes to detect homology to sequences that code for Shiga-like toxins I and II; heat-stabile and heat-labile toxins, adherence factors EAF and eae, and the fimbrial antigen of enterohemorrbagic E. coli. Fifty-nine (13%) of the isolates demonstrated homology with one or more specific DNA probes. More than 50% of the isolates in treated water were not recovered in MMO-4-methylumbelliferyl-beta-D-glucuronide media designed for detection of this indicator.

引用

页码：3095 / 3100

页数：6

共 38 条

[1] SEROTYPES OF ESCHERICHIA-COLI THAT HYBRIDIZED WITH DNA PROBES FOR GENES ENCODING SHIGA-LIKE TOXIN-I, SHIGA-LIKE TOXIN-II, AND SEROGROUP-O157 ENTEROHEMORRHAGIC ESCHERICHIA-COLI FIMBRIAE ISOLATED FROM ADULTS WITH DIARRHEA IN THAILAND [J].