ISOLATED BASAL KERATINOCYTES EXPRESS PEMPHIGOID GESTATIONIS ANTIGEN

被引：4

作者：

KELLY, SE

MCVITTIE, E

BLACK, MM

FLEMING, S

机构：

[1] UNIV EDINBURGH, DEPT PATHOL, EDINBURGH EH3 9YW, MIDLOTHIAN, SCOTLAND

[2] ST THOMAS HOSP, DOWLING SKIN UNIT, LONDON SE1 7EH, ENGLAND

来源：

BRITISH JOURNAL OF DERMATOLOGY | 1992年 / 126卷 / 01期

关键词：

D O I：

10.1111/j.1365-2133.1992.tb08401.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Basal keratinocytes were isolated from epidermal cell suspensions prepared by trypsinization of normal human skin. Cells were identified as basal cells by their adherence to collagen and confirmed as basal cells by the presence of pemphigoid antigen. Using an indirect immunofluorescence assay, cells were found to express pemphigoid gestationis-related antigen. Sera from patients with pemphigoid gestationis reacted in one of two immunofluorescence patterns: either polar, in a pattern similar to that observed with bullous pemphigoid serum. or with uniform staining around the cell periphery. Pemphigoid gestationis-related antigen is expressed by isolated basal keratinocytes and is resistant to trypsinization. The heterogeneity of immunofluorescence patterns may correspond to the heterogeneity of antigen recognition by different patients with pemphigoid gestationis.

引用

收藏

页码：42 / 46

页数：5

相关论文

共 18 条

[1] ABNORMAL EXPRESSION OF CLASS-II MHC ANTIGENS IN PLACENTAE FROM PATIENTS WITH PEMPHIGOID GESTATIONIS - ANALYSIS OF CLASS-II MHC SUBREGION PRODUCT EXPRESSION [J].

BORTHWICK, GM ;

HOLMES, RC ;

STIRRAT, GM .

PLACENTA, 1988, 9 (01) :81-94

[2] DIFFERENTIAL BINDING OF ANTIBODY FROM SERA OF PATIENTS WITH PEMPHIGUS AND PEMPHIGOID TO ISOLATED GUINEA-PIG EPIDERMAL-CELLS [J].

BRIGDEN, WD ;

AMOS, HE .

BRITISH JOURNAL OF DERMATOLOGY, 1975, 93 (04) :425-430

[3] RECOGNITION OF 2 DISTINCT MAJOR ANTIGENS BY BULLOUS PEMPHIGOID SERA [J].

COOK, AL ;

HANAHOE, THP ;

MALLETT, RB ;

PYE, RJ .

BRITISH JOURNAL OF DERMATOLOGY, 1990, 122 (04) :435-444

[4] ISOLATION OF A HUMAN EPIDERMAL CDNA CORRESPONDING TO THE 180-KD AUTOANTIGEN RECOGNIZED BY BULLOUS PEMPHIGOID AND HERPES-GESTATIONIS SERA - IMMUNOLOCALIZATION OF THIS PROTEIN TO THE HEMIDESMOSOME [J].

DIAZ, LA ;

RATRIE, H ;

SAUNDERS, WS ;

FUTAMURA, S ;

SQUIQUERA, HL ;

ANHALT, GJ ;

GIUDICE, GJ .

JOURNAL OF CLINICAL INVESTIGATION, 1990, 86 (04) :1088-1094

[5] SOME BIOCHEMICAL-PROPERTIES OF PEMPHIGOID ANTIGEN BOUND TO THE SURFACE OF DISSOCIATED EPIDERMAL BASAL CELLS [J].

GREKIN, PM ;

LEVY, GN ;

KING, AJ ;

DIAZ, LA .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1981, 76 (03) :190-192

[6] C3 LEUKOTACTIC FACTORS PRODUCED BY A TISSUE PROTEASE [J].

HILL, JH ;

WARD, PA .

JOURNAL OF EXPERIMENTAL MEDICINE, 1969, 130 (03) :505-&

[7] IMMUNOPATHOLOGY OF THE PLACENTA IN PEMPHIGOID GESTATIONIS AND LINEAR IGA DISEASE [J].

KELLY, SE ;

FLEMING, S ;

BHOGAL, BS ;

WOJNAROWSKA, F ;

BLACK, MM .

BRITISH JOURNAL OF DERMATOLOGY, 1989, 120 (06) :735-743

[8] THE DISTRIBUTION OF IGG SUBCLASSES IN PEMPHIGOID GESTATIONIS - PG FACTOR IS AN IGG1 AUTOANTIBODY [J].

KELLY, SE ;

CERIO, R ;

BHOGAL, BS ;

BLACK, MM .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1989, 92 (05) :695-698

[9] EXPRESSION OF A PEMPHIGOID GESTATIONIS-RELATED ANTIGEN BY HUMAN-PLACENTA [J].

KELLY, SE ;

BHOGAL, BS ;

WOJNAROWSKA, F ;

BLACK, MM .

BRITISH JOURNAL OF DERMATOLOGY, 1988, 118 (05) :605-611

[10] WESTERN-BLOT-ANALYSIS OF THE ANTIGEN IN PEMPHIGOID GESTATIONIS [J].

KELLY, SE ;

BHOGAL, BS ;

WOJNAROWSKA, F ;

WHITEHEAD, P ;

LEIGH, IM ;

BLACK, MM .

BRITISH JOURNAL OF DERMATOLOGY, 1990, 122 (04) :445-449