ISOLATION OF A GENE EXPRESSED DURING SUCROSE ACCUMULATION IN LEAVES OF LOLIUM-TEMULENTUM L

被引：12

作者：

WINTERS, AL

GALLAGHER, J

POLLOCK, CJ

FARRAR, JF

机构：

[1] INST GRASSLAND & ENVIRONM RES,DEPT CELL BIOL,ABERYSTWYTH SY23 3EB,DYFED,WALES

[2] UNIV COLL N WALES,SCH BIOL SCI,BANGOR LL57 2UW,GWYNEDD,WALES

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 1995年 / 46卷

关键词：

LEAF; SUCROSE; GENE EXPRESSION; S-ADENOSYLMETHIONINE SYNTHETASE;

D O I：

10.1093/jxb/46.special_issue.1345

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A 1620 base pair cDNA clone showing increased expression during sucrose accumulation in leaves of Lolium temulentum has been isolated by differential screening. Comparison of the DNA sequence of the open reading frame with published sequences has revealed extensive homology with sequences coding for ATP:L-methionine S-adenosylmethionine synthetase, EC 2.5.1.6 (SAM synthetase) (79.6% with Arabidopsis thaliana and 81.6% with Dianthus caryophyllus). Increased expression was observed in both intact seedling leaves and detached mature leaves during sucrose accumulation. Expression decreased during sucrose mobilization in leaves of intact seedlings. The significance of increased expression of a gene coding for SAM synthetase during sucrose accumulation is discussed in relation to the involvement of sucrose in the regulation of plant metabolism.

引用

页码：1345 / 1350

页数：6

共 29 条

[1] COMPLEMENTATION OF SNF1, A MUTATION AFFECTING GLOBAL REGULATION OF CARBON METABOLISM IN YEAST, BY A PLANT PROTEIN-KINASE CDNA [J].