INSERTION OF IS4 INTO THE TNPA GENE OF TN3 - SEQUENCING OF THE INSERTION SITE

被引：2

作者：

MORITA, M ^{[1
]}

SUGINO, Y ^{[1
]}

机构：

[1] KANSAI MED UNIV,BIOCHEM LAB,18-89 UYAMA HIGASHI,HIRAKATA,OSAKA 573,JAPAN

来源：

GENE | 1990年 / 96卷 / 01期

关键词：

direct repeat; Recombinant DNA; target-site specificity; transposase;

D O I：

10.1016/0378-1119(90)90352-R

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Plasmid pMM237 lacks the deletion-forming activity associated with its Tn3 transposase-encoding gene (tnpA) [Morita et al., J. Biochem. 101 (1987) 1253-1264]. Analysis of the plasmid DNA showed that pMM237 was generated by insertion of IS4 into the tnpA gene of the original plasmid pMM234. The insertion was accompanied by a direct 12-bp duplication at the target site. The nucleotide sequence around the target site shared only some of the characteristics of previously reported IS4 target sites [Habermann et al., Mol. Gen. Genet. 175 (1979) 369-373; Klaer et al., Mol. Gen. Genet. 181 (1981) 169-175; Georgopoulos et al., Gene 20 (1982) 83-90; Mayaux et al., Gene 30 (1984) 137-146]. © 1990.

引用

页码：129 / 132

页数：4

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