GENETICALLY ENGINEERED RICE RESISTANT TO RICE STRIPE VIRUS, AN INSECT-TRANSMITTED VIRUS

被引：112

作者：

HAYAKAWA, T ^{[1
]}

ZHU, YF ^{[1
]}

ITOH, K ^{[1
]}

KIMURA, Y ^{[1
]}

IZAWA, T ^{[1
]}

SHIMAMOTO, K ^{[1
]}

TORIYAMA, S ^{[1
]}

机构：

[1] NATL INST AGROENVIRONM SCI,TSUKUBA 305,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 20期

关键词：

TRANSGENIC RICE; COAT PROTEIN; VIRUS RESISTANCE;

D O I：

10.1073/pnas.89.20.9865

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The coat protein (CP) gene of rice stripe virus was introduced into two japonica varieties of rice by electroporation of protoplasts. The resultant transgenic plants expressed the CP at high levels (up to 0.5% of total soluble protein) and exhibited a significant level of resistance to virus infection. Plants derived from selfed progeny of the primary transformants also expressed the CP and showed viral resistance, indicating stable transmission of the CP gene and the trait of resistance to the next generation. Moreover, the virally encoded stripe disease-specific protein was not detected in transgenic plants expressing CP 8 weeks after inoculation, indicating protection before viral multiplication. These studies demonstrated that CP-mediated resistance to virus infection can be extended to cereals and to the viruses transmitted by an insect vector (planthopper).

引用

页码：9865 / 9869

页数：5

共 49 条

[1] HISTOCHEMICAL ANALYSIS OF CAMV 35S PROMOTER-BETA-GLUCURONIDASE GENE-EXPRESSION IN TRANSGENIC RICE PLANTS [J].