LABORATORY PRODUCTION OF OOSPORES OF PERONOSPORA-PARASITICA (CRUCIFER DOWNY MILDEW) AND THE RECOVERY AND CHARACTERIZATION OF SEXUAL PROGENY FROM CROSSES BETWEEN ISOLATES WITH DIFFERENT HOST-SPECIFICITY

被引:19
作者
MOSS, NA
CRUTE, IR
LUCAS, JA
机构
[1] UNIV NOTTINGHAM,DEPT LIFE SCI,NOTTINGHAM NG7 2RD,ENGLAND
[2] HORT RES INT,WELLESBOURNE CV35 9EF,WARWICK,ENGLAND
关键词
D O I
10.1111/j.1365-3059.1994.tb01611.x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The production of viable oospores of Peronospora parasitica under laboratory conditions and the recovery of isolates (referred to as sexual progeny) from these oospore populations are described. Oospores were produced when isolates of opposite sexual compatibility type, specialized to the same or different Brassica species, were grown together in seedling cotyledons of a host line capable of supporting growth of both isolates. Recovery of sexual progeny from oospore populations produced from two out of four pairings between isolates specialized to the same host species (homologous pairings) proved relatively easy. On the basis of their characterization with respect to virulence, response to phenylamide fungicides, sexual compatibility type and isoenzyme polymorphisms, there was evidence that the sexual progeny from these homologous pairings could be of hybrid origin. For the first time in a member of the Peronosporaceae, it proved possible to recover and successfully characterize a few sexual progeny from pairings between isolates specialized to different host species (heterologous pairings). However, the majority of such isolates sporulated weakly and as a consequence proved difficult to maintain and were lost. Nevertheless, some evidence for the hybrid nature of progeny from heterologous pairings was obtained.
引用
收藏
页码:713 / 725
页数:13
相关论文
共 27 条
[1]  
AUSTIN RB, 1972, PHOTOSYNTHETICA, V6, P41
[2]  
Channon A., 1981, DOWNY MILDEWS, P321
[3]   STUDIES ON THE INHERITANCE OF RESISTANCE TO METALAXYL IN BREMIA-LACTUCAE AND ON THE STABILITY AND FITNESS OF FIELD ISOLATES [J].
CRUTE, IR ;
HARRISON, JM .
PLANT PATHOLOGY, 1988, 37 (02) :231-250
[4]   THE OCCURRENCE, CHARACTERISTICS AND DISTRIBUTION IN THE UNITED-KINGDOM OF RESISTANCE TO PHENYLAMIDE FUNGICIDES IN BREMIA-LACTUCAE (LETTUCE DOWNY MILDEW) [J].
CRUTE, IR ;
NORWOOD, JM ;
GORDON, PL .
PLANT PATHOLOGY, 1987, 36 (03) :297-315
[5]  
CRUTE IR, 1993, ADV MOL GENETICS PLA, V2, P437
[6]  
Dangl J. L., 1992, Methods in Arabidopsis research., P393
[7]   HOST RANGE AND TAXONOMY OF PERONOSPORA ON CRUCIFERS [J].
DICKINSON, CH ;
GREENHALGH, JR .
TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1977, 69 (AUG) :111-116
[8]   PHYSICAL, ENZYMATIC, AND CHEMICAL FACTORS AFFECTING VIABILITY AND GERMINATION OF OOSPORES OF PHYTOPHTHORA-MEGASPERMA F-SP MEDICAGINIS [J].
ELHAMALAWI, ZA ;
ERWIN, DC .
PHYTOPATHOLOGY, 1986, 76 (05) :503-507
[9]  
Gordon P.L, 1985, BCPC MONOGRAPH, V31, P311
[10]  
HOLUB E, 1993, APS SYMP SER, P21