INVOLVEMENT OF DNASE-II IN NUCLEAR DEGENERATION DURING LENS CELL-DIFFERENTIATION

The characterization of DNase II and DNase I activity was undertaken to discriminate their different roles in physiological nuclear degradation during lens fiber cell differentiation. The activity of both nucleases determined in a new assay allows to discriminate DNase II from DNase I in the same extract, In fibers, both types of nuclease activities are found and appear higher than in epithelial cells. Specific polyclonal antibodies directed against these two nucleases reveal by Western blot analysis the presence of various DNase isoforms, DNase II like-nuclease, present in fibers, is represented by three major bands (60, 23, and 18 kDa), which are not detected, at least for two of them (60 and 23 kDa), in epithelial cells, DNase I like nuclease pattern in fiber cells shows a single 32-kDa band, while several bands can be detected in epithelial cells, Immunocytochemistry studies show both nucleases present in lens cell sections, DNase II is, as usual, in cytoplasm of epithelial cells, but it appears strikingly concentrated in the nuclei of fibers, DNase I is always concentrated in nuclei of epithelial and fiber cells, DNA degradation observed in agarose gels shows that DNase II-activating medium cleaves the DNA from fiber cells more efficiently than DNase I-activating buffer, In addition, DNase II antibody is able to prevent this degradation, These results suggest a specific involvement of DNase II in nuclear degradation during lens cell differentiation.