RAPID SCREENING FOR RESISTANCE TO GANCICLOVIR AND FOSCARNET OF PRIMARY ISOLATES OF HUMAN CYTOMEGALOVIRUS FROM CULTURE-POSITIVE BLOOD-SAMPLES

被引：36

作者：

GERNA, G

SARASINI, A

PERCIVALLE, E

ZAVATTONI, M

BALDANTI, F

REVELLO, MG

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 03期

关键词：

D O I：

10.1128/JCM.33.3.738-741.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid screening assay for the detection of resistance to ganciclovir and foscarnet of primary isolates of human cytomegalovirus from culture-positive blood samples was developed by using single doses of both drugs and an immediate-early antigen plaque reduction assay. Results of the rapid assay with peripheral blood leukocytes as the viral inoculum were compared with those of a conventional assay with cell-free virus from the relevant viral isolates. Both assays gave overlapping results. The rapid assay offers the following major advantages: (i) it provides results within 4 to 6 days, (ii) it can be performed with peripheral blood leukocytes, and (iii) it reliably detects drug-resistant human cytomegalovirus strains.

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页码：738 / 741

页数：4

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