SPECTROSCOPIC AND ELECTROCHEMICAL CHARACTERIZATION OF CHLOROPHYLL-A IN DIFFERENT WATER + ORGANIC-SOLVENT MIXTURES

被引：25

作者：

AGOSTIANO, A

COSMA, P

DELLAMONICA, M

机构：

[1] Dipartimento di Chimica, Bari

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1990年 / 23卷 / 03期

关键词：

D O I：

10.1016/0302-4598(90)80019-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Triangular-sweep adsorption voltammetry and absorption and fluorescence spectroscopy are employed in the study of chlorophyll a hydration and aggregation in binary mixtures of water with acetone, dimethylformamide (DMF) and acetonitrile (ACN). From the variation of the relative intensities and positions of the polarographic and spectroscopic peaks with the composition of the solvent, it is concluded that the organic solvates of Chl a are completely converted to Chl a·2 H2O at a water mole fraction χH2O ≫ 0.66. A further shift in the equilibrium towards the dimerization of Chl a·2 H2O is observed at χH2O ≫ 0.75. The lower water content and Chl a concentration required for aggregation in DMF and ACN compared with that required in acetone is discussed in terms of the bulk properties of the binary solvent mixtures. © 1990.

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页码：311 / 324

页数：14

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