SITE-SPECIFIC INTEGRATION OF THE HAEMOPHILUS-INFLUENZAE BACTERIOPHAGE-HP1 - LOCATION OF THE BOUNDARIES OF THE PHAGE ATTACHMENT SITE

被引：23

作者：

HAUSER, MA ^{[1
]}

SCOCCA, JJ ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT BIOCHEM,615 N WOLFE ST,BALTIMORE,MD 21205

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 20期

关键词：

D O I：

10.1128/JB.174.20.6674-6677.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasmids containing DNA segments from the attachment region of phage HP1 were constructed and tested for the ability to replace the phage attachment site substrate in site-specific recombination reactions. The distance separating the boundaries of the functional site was 418 bp. Replacements within the 11-residue segment 5'-GGCGGTTATCG at the left boundary or within the 12-residue segment 5'-GGATTTTTTGAA at the right boundary abolished substrate activity. A segment of the 418-residue sequence preserves the integrity of an operon of three Haemophilus influenzae tRNA genes after HP1 insertion within the coding sequence.

引用

页码：6674 / 6677

页数：4

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