REGULATION OF ALTERNATIVE OXIDASE KINETICS BY PYRUVATE AND INTERMOLECULAR DISULFIDE BOND REDOX STATUS IN SOYBEAN SEEDLING MITOCHONDRIA

被引：136

作者：

UMBACH, AL

WISKICH, JT

SIEDOW, JN

机构：

[1] DUKE UNIV,DEPT BOT,DURHAM,NC 27706

[2] UNIV ADELAIDE,DEPT BOT,ADELAIDE,SA 5001,AUSTRALIA

来源：

FEBS LETTERS | 1994年 / 348卷 / 02期

关键词：

CYANIDE-RESISTANT OXIDASE; PLANT MITOCHONDRION; PYRUVATE ACTIVATION; DIMERIC ENZYME; REGULATORY DISULFIDE BOND;

D O I：

10.1016/0014-5793(94)00600-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two factors known to regulate plant mitochondrial cyanide-resistant alternative oxidase activity, pyruvate and the redox status of the enzyme's intermolecular disulfide bond, were shown to differently affect activity in isolated soybean seedling mitochondria. Pyruvate stimulated alternative oxidase activity at low levels of reduced ubiquinone, shifting the threshold level of ubiquinone reduction for enzyme activity to a lower value. The disulfide bond redox status determined the maximum enzyme activity obtainable in the presence of pyruvate, with the highest rates occurring when the bond was reduced. With variations in cellular pyruvate levels and in the proportion of reduced alternative oxidase protein, a wide range of enzyme activity is possible in vivo.

引用

页码：181 / 184

页数：4

共 16 条

[1] REGULATION OF ALTERNATIVE PATHWAY ACTIVITY IN PLANT-MITOCHONDRIA - NONLINEAR RELATIONSHIP BETWEEN ELECTRON FLUX AND THE REDOX POISE OF THE QUINONE POOL [J].