2 KINDS OF 2-HALO ACID DEHALOGENASES FROM PSEUDOMONAS SP YL INDUCED BY 2-CHLOROACRYLATE AND 2-CHLOROPROPIONATE

被引：29

作者：

HASAN, AKMQ ^{[1
]}

TAKADA, H ^{[1
]}

KOSHIKAWA, H ^{[1
]}

LIU, JQ ^{[1
]}

KURIHARA, T ^{[1
]}

ESAKI, N ^{[1
]}

SODA, K ^{[1
]}

机构：

[1] KYOTO UNIV, INST CHEM RES, MICROBIAL BIOCHEM LAB, UJI, KYOTO 611, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 09期

关键词：

D O I：

10.1271/bbb.58.1599

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bacterial isolates WS, WL, and YL, which were identified as Pseudomonas, grew in 2-chloro-acrylate (2-CAA) and 2-chloropropionate (2-CPA) media to produce 2-halo acid dehalogenases. Pseudomonas sp. YL showed the highest 2-CPA dehalogenase activity among the three strains, and inducibly produced two kinds of 2-halo acid dehalogenases in a medium containing either 2-CAA or 2-CPA. The 2-CAA-induced enzyme catalyzed dehalogenation of D- and L-2-CPA to produce L- and D-lactate, respectively. Its pH optimum of the dehalogenation and activity staining indicated that a single enzyme catalyzes the dehalogenation of both enantiomers of 2-CPA. The 2-CPA-induced enzyme catalyzed production of D-lactate from L-2-CPA, but did not act on D-2-CPA. The two dehalogenases are different from each other in mobility upon polyacrylamide gel electrophoresis and sensitivity towards thiol reagents.

引用

页码：1599 / 1602

页数：4

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