ASSIGNMENT OF THE REDOX POTENTIALS TO THE 4 HEMES IN DESULFOVIBRIO-VULGARIS CYTOCHROME-C(3) BY 2D-NMR

被引：76

作者：

SALGUEIRO, CA

TURNER, DL

SANTOS, H

LEGALL, J

XAVIER, AV ^{[1
]}

机构：

[1] CTR TECNOL QUIM & BIOL,APT 127,P-2780 OEIRAS,PORTUGAL

[2] UNIV NOVA LISBOA,P-1200 LISBON,PORTUGAL

[3] UNIV SOUTHAMPTON,DEPT CHEM,SOUTHAMPTON SO9 5NH,HANTS,ENGLAND

[4] UNIV GEORGIA,DEPT BIOCHEM,ATHENS,GA 30602

来源：

FEBS LETTERS | 1992年 / 314卷 / 02期

关键词：

CYTOCHROME-C(3); MULTIHAEM PROTEIN; 2D-NMR; ROESY; NOESY; X-RAY STRUCTURE;

D O I：

10.1016/0014-5793(92)80963-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using 2D-NMR the four haems of Desulfovibrio vulgaris (Hildenborough) cytochrome c3 within the X-ray structure were fully cross assigned according to their redox potential. The strategy used was based on a complete network of chemical exchange connectivities between the NMR signals obtained for all oxidation levels to the corresponding ones in the fully reduced spectrum [1992, Eur. J. Biochem., in press]. This unequivocal cross-assignment disagrees with earlier results obtained for the similar protein from Desulfovibrio vulgaris (Miyazaki F.) [1991, FEBS Lett. 285, 149-151].

引用

收藏

页码：155 / 158

页数：4

相关论文

共 20 条

[1] EPR STUDY OF THE REDOX INTERACTIONS IN CYTOCHROME-C3 FROM DESULFOVIBRIO-VULGARIS MIYAZAKI [J].

BENOSMAN, H ;

ASSO, M ;

BERTRAND, P ;

YAGI, T ;

GAYDA, JP .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (01) :51-55

[2] STRUCTURE DETERMINATION OF A TETRASACCHARIDE - TRANSIENT NUCLEAR OVERHAUSER EFFECTS IN THE ROTATING FRAME [J].

BOTHNERBY, AA ;

STEPHENS, RL ;

LEE, JM ;

WARREN, CD ;

JEANLOZ, RW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (03) :811-813

[3] A THERMODYNAMIC MODEL FOR THE COOPERATIVE FUNCTIONAL-PROPERTIES OF THE TETRAHEME CYTOCHROME C3 FROM DESULFOVIBRIO-GIGAS [J].

COLETTA, M ;

CATARINO, T ;

LEGALL, J ;

XAVIER, AV .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 202 (03) :1101-1106

[4] REVISION OF THE HEME-CORE ARCHITECTURE IN THE TETRAHEAM CYTOCHROME C3 FROM DESULFOVIBRIO-BACULATUS BY 2-DIMENSIONAL H-1-NMR [J].

COUTINHO, IB ;

TURNER, DL ;

LEGALL, J ;

XAVIER, AV .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 209 (01) :329-333

[5] OUTLINE STRUCTURE OF CYTOCHROME C3 AND CONSIDERATION OF ITS PROPERTIES [J].

DOBSON, CM ;

HOYLE, NJ ;

GERALDES, CF ;

BRUSCHI, M ;

LEGALL, J ;

WRIGHT, PE ;

WILLIAMS, RJP .

NATURE, 1974, 249 (5456) :425-429

[6] STRUCTURAL ASSIGNMENT OF THE HEME POTENTIALS OF CYTOCHROME-C3, USING A SPECIFICALLY MODIFIED ARGININE [J].

DOLLA, A ;

CAMBILLAU, C ;

BIANCO, P ;

HALADJIAN, J ;

BRUSCHI, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 147 (02) :818-823

[7] ESTIMATION OF MICROSCOPIC REDOX POTENTIALS OF A TETRAHEME PROTEIN, CYTOCHROME-C3 OF DESULFOVIBRIO-VULGARIS, MIYAZAKI-F, AND PARTIAL ASSIGNMENTS OF HEME GROUPS [J].

FAN, K ;

AKUTSU, H ;

KYOGOKU, Y ;

NIKI, K .

BIOCHEMISTRY, 1990, 29 (09) :2257-2263

[8] SINGLE-CRYSTAL ELECTRON-PARAMAGNETIC RESONANCE STUDY OF CYTOCHROME-C3 FROM DESULFOVIBRIO-DESULFURICANS NORWAY STRAIN - ASSIGNMENT OF THE HEME MIDPOINT REDOX POTENTIALS [J].

GUIGLIARELLI, B ;

BERTRAND, P ;

MORE, C ;

HASER, R ;

GAYDA, JP .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 216 (01) :161-166

[9] REFINED STRUCTURE OF CYTOCHROME-C3 AT 1.8 A RESOLUTION [J].

HIGUCHI, Y ;

KUSUNOKI, M ;

MATSUURA, Y ;

YASUOKA, N ;

KAKUDO, M .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 172 (01) :109-139

[10]

LeGall J., 1988, BIOL ANAEROBIC MICRO, P587