SECRETION OF TRANSFORMING GROWTH-FACTOR-BETA BY THE IMMATURE RAT PROSTATE

被引：10

作者：

ATFI, A ^{[1
]}

SAMPEREZ, S ^{[1
]}

JOUAN, P ^{[1
]}

机构：

[1] FAC MED PHARM RENNES, CTR REG RECH ENDOCRINOL, RENNES, FRANCE

来源：

PROSTATE | 1994年 / 24卷 / 03期

关键词：

TGF-BETA; PROSTATE; INHIBITORY FACTOR;

D O I：

10.1002/pros.2990240309

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Immature rat ventral prostate was used to identify a potent cell growth inhibitory factor in normal prostate. Both conditioned media and peptide extracts derived from ventral prostates inhibit the cellular growth and DNA synthesis in metastatic androgen-independent human prostate carcinoma cell line (PC3). The prostatic inhibitory factor was partially purified using an hydrophobic HPLC column (C18). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis (SDS-PAGE) of partially purified material revealed a major 25-kDa band. Immunoblot analysis showed similarity of the inhibitory factor to the transforming growth factor-beta (TGF-beta). Furthermore, the proliferation inhibiting activity of the prostatic factor was completely abolished by anti-TGF-beta antibodies. These data indicate that normal prostate produces a TGF-beta-like protein under non-physiological conditions. (C) 1994 Wiley-Liss, Inc.

引用

页码：149 / 155

页数：7

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