EFFICIENT PLANT-REGENERATION FROM INDICA (GROUP-1) RICE PROTOPLASTS OF ONE ADVANCED BREEDING LINE AND 3 VARIETIES

被引：14

作者：

ZHANG, SP ^{[1
]}

机构：

[1] INT RICE RES INST, TISSUE CULTURE LAB, MANILA 1099, PHILIPPINES

来源：

PLANT CELL REPORTS | 1995年 / 15卷 / 1-2期

关键词：

INDICA RICE; PLANT REGENERATION; PROTOPLASTS;

D O I：

10.1007/BF01690256

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Regenerable embryogenic suspensions were established from one Indica (group 1) rice advanced breeding line and 9 Indica (group 1) rice varieties in 6-8 weeks. Four were chosen for protoplast culture and plant regeneration. About 4-7x10(7) protoplasts were isolated from one gram of 8-week-old cell suspension. High plating efficiency (30.5%) and colony formation (13.7%) were obtained using nurse culture methods. A high plant regeneration frequency (67.5%) was observed for line IR57311-95-2-3. In total, 322 plants were regenerated. All the regenerated plants were fertile.

引用

页码：68 / 71

页数：4

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