DETERMINATION OF LACTATE-DEHYDROGENASE BY THE ELECTROCHEMICAL OXIDATION OF NADH AT A MODIFIED MICROBAND GOLD ELECTRODE

被引：9

作者：

CAI, CX

JU, HX

CHEN, HY

机构：

[1] Department of Chemistry, Nanjing University

来源：

ANALYTICAL LETTERS | 1995年 / 28卷 / 05期

关键词：

AMPEROMETRY; LACTATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; MICROELECTRODE; CHEMICALLY MODIFIED ELECTRODE;

D O I：

10.1080/00032719508001426

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new amperometric electrode for the assay of lactate dehydrogenase (LDH) is prepared by modifying a nanometer-sized microband gold electrode with nickel hexacyanoferrate. The amperometric determination bases on electrochemically detecting NADH produced by the enzymatic reaction of lactate with NAD(+) under the catalysis of LDH. The modified electrode can sensitively detect LDH at +0.55V vs. SCE with a linear response in the range of 10U/mL to 350/mL under optimum conditions.

引用

页码：809 / 820

页数：12

共 16 条

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