PHYSIOLOGICAL CONCENTRATIONS OF MELATONIN INHIBIT NITRIC-OXIDE SYNTHASE IN RAT CEREBELLUM

被引：193

作者：

POZO, D

REITER, RJ

CALVO, JR

GUERRERO, JM

机构：

[1] UNIV SEVILLE, SCH MED, DEPT MED BIOCHEM & MOLEC BIOL, E-41009 SEVILLE, SPAIN

[2] UNIV TEXAS, HLTH SCI CTR, DEPT CELLULAR & STRUCT BIOL, SAN ANTONIO, TX 78284 USA

来源：

LIFE SCIENCES | 1994年 / 55卷 / 24期

关键词：

MELATONIN; PINEAL GLAND; NITRIC OXIDE; CEREBELLUM;

D O I：

10.1016/0024-3205(94)00532-X

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

In the present paper we show the inhibitory effect of melatonin on rat cerebellar nitric oxide synthase (NOS) activity. NO production was monitored by the stoichiometric conversion of L-arginine to L-citrulline. The inhibitory effect of melatonin was dose-dependent, with an IC50 value of about 0.1 mM. However, a significant inhibition of enzyme activity (>22%) was observed at 1 nM melatonin which is in the range of the physiological serum concentration of the hormone at night. The inhibitory effect of melatonin was observed exclusively in the presence of Ca++. Results suggest a new and important role of the pineal hormone melatonin on central nervous system processes, i.e., by modulating NO production.

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收藏

页码：PL455 / PL460

页数：6

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