TYPING OF EPIDEMIOLOGICALLY ASSOCIATED ENVIRONMENTAL AND CLINICAL STRAINS OF LISTERIA-MONOCYTOGENES BY RANDOM AMPLIFICATION OF POLYMORPHIC DNA

被引：27

作者：

KERR, KG

KITE, P

HERITAGE, J

HAWKEY, PM

机构：

[1] Department of Microbiology, University of Leeds, Old Medical School, Leeds, LS2 9JT, Thoresby Place

来源：

JOURNAL OF FOOD PROTECTION | 1995年 / 58卷 / 06期

关键词：

LISTERIA MONOCYTOGENES; PCR TYPING; RAPD;

D O I：

10.4315/0362-028X-58.6.609

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

One hundred and three strains of Listeria monocytogenes (Lm), Listeria seeligeri and Listeria innocua of clinical, food, and environmental origin were examined by generating randomly amplified polymorphic DNA (RAPD). Using one 10 bp and two 13 bp random primers, epidemiologically related strains, previously shown to be indistinguishable by phage typing, yielded identical RAPD profiles. Strains isolated from the hands of three workers in a retail food establishment showed the presence of a single predominant Lm isolate. RAPD analysis is a rapid, reproducible, and relatively inexpensive method for the differentiation of epidemiologically linked isolates of Listeria spp.

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页码：609 / 613

页数：5

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