STUDY OF O-GLYCAN SIALYLATION IN C6 CULTURED GLIOMA-CELLS - EVIDENCE FOR POSTTRANSLATIONAL REGULATION OF A BETA-GALACTOSIDE-ALPHA-2,3 SIALYLTRANSFERASE ACTIVITY BY N-GLYCOSYLATION

被引：9

作者：

BROQUET, P ^{[1
]}

GEORGE, P ^{[1
]}

GEOFFROY, J ^{[1
]}

REBOUL, P ^{[1
]}

LOUISOT, P ^{[1
]}

机构：

[1] FAC MED LYON SUD,BIOCHIM GEN & MED LAB,CNRS,INSERM,U189,BP 12,F-69921 OULLINS,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91054-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the Galβ1-3GalNAc-R α 2,3 sialyltransferase from C6 glioma cells transferring Neu5Ac from CMP-Neu5Ac onto O-glycans of glycoproteins. Using synchronized C6 glioma cells, we showed that the α2,3 sialyltransferase activity was inhibited by tunicamycin to a greater extend than DNA and protein biosynthesis suggesting inhibition of N-glycosylation of this enzyme. Additional demonstration of N-glycosylation of the α2,3 sialyltransferase was provided through ConA-Sepharose binding. Treatment of partially purified α2,3 sialyltransferase by peptide-N-glycosidase F showed a significative inhibition demonstrating that N-glycan moiety is required for complete activity of the C6 glioma cell α2,3 sialyltransferase. © 1991.

引用

页码：1437 / 1443

页数：7

共 23 条

[1]

BARONDES SH, 1970, BRAIN GLYCOCONJUGATE, P747

[2] EVIDENCE FOR AN O-GLYCAN SIALYLATION SYSTEM IN BRAIN - CHARACTERIZATION OF A BETA-GALACTOSIDE ALPHA-2,3-SIALYLTRANSFERASE FROM RAT-BRAIN REGULATING THE EXPRESSION OF AN ALPHA-N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE ACTIVITY [J].

BAUBICHONCORTAY, H ;

BROQUET, P ;

GEORGE, P ;

LOUISOT, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (02) :257-265

[3] DIFFERENT REACTIVITY TO LYSOPHOSPHATIDYLCHOLINE, DIDS AND TRYPSIN OF 2 BRAIN SIALYLTRANSFERASES SPECIFIC FOR OMICRON-GLYCANS - A CONSEQUENCE OF THEIR TOPOGRAPHY IN THE ENDOPLASMIC MEMBRANES [J].

BAUBICHONCORTAY, H ;

SERRESGUILLAUMOND, M ;

BROQUET, P ;

LOUISOT, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 862 (02) :243-253

[4] DIFFERENT REACTIVITY OF 2 BRAIN SIALYLTRANSFERASES TOWARDS SULFHYDRYL-REAGENTS - EVIDENCE FOR A THIOL-GROUP INVOLVED IN THE NUCLEOTIDE-SUGAR BINDING-SITE OF THE NEUAC-ALPHA-2-3GAL-BETA-1-3GALNAC ALPHA-(2-6)SIALYLTRANSFERASE [J].

BAUBICHONCORTAY, H ;

BROQUET, P ;

GEORGE, P ;

LOUISOT, P .

GLYCOCONJUGATE JOURNAL, 1989, 6 (01) :115-127

[5]

BAUBICHONCORTAY H, 1986, CARBOHYD RES, V146, P209

[6] DIFFERENTIATED RAT GLIAL CELL STRAIN IN TISSUE CULTURE [J].

BENDA, P ;

LIGHTBODY, J ;

SATO, G ;

LEVINE, L ;

SWEET, W .

SCIENCE, 1968, 161 (3839) :370-+

[7] ENZYMOLOGY OF MYELINATION [J].

BRADY, RO ;

QUARLES, RH .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 1973, 2 (01) :23-29

[8] BIOSYNTHESIS OF CEREBRAL GLYCOPROTEINS - STUDIES ON MITOCHONDRIAL MANNOSYL TRANSFERASE [J].

BROQUET, P ;

MORELIS, R ;

LOUISOT, P .

JOURNAL OF NEUROCHEMISTRY, 1975, 24 (05) :989-&

[9] EFFECT OF DESIPRAMINE ON A GLYCOPROTEIN SIALYLTRANSFERASE ACTIVITY IN C6 CULTURED GLIOMA-CELLS [J].

BROQUET, P ;

BAUBICHONCORTAY, H ;

GEORGE, P ;

PESCHARD, MJ ;

LOUISOT, P .

JOURNAL OF NEUROCHEMISTRY, 1990, 54 (02) :388-394

[10] ASSAY FOR NANOGRAM QUANTITIES OF DNA IN CELLULAR HOMOGENATES [J].

BRUNK, CF ;

JONES, KC ;

JAMES, TW .

ANALYTICAL BIOCHEMISTRY, 1979, 92 (02) :497-500

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