LOW-MOLECULAR-WEIGHT FACTOR-X ACTIVATOR FROM CERASTES-VIPERA (SAHARA SAND VIPER) VENOM

被引：16

作者：

FARID, T ^{[1
]}

NASSER, H ^{[1
]}

ZAKI, K ^{[1
]}

ELASMAR, MF ^{[1
]}

机构：

[1] AIN SHAMS UNIV, FAC MED, DEPT BIOCHEM, RES UNIT NAT TOXINS, CAIRO, EGYPT

来源：

TOXICON | 1993年 / 31卷 / 08期

关键词：

D O I：

10.1016/0041-0101(93)90260-P

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Fraction G from Cerastes vipera venom previously purified on Sephadex G100 was refractionated on DEAE-Sephadex A50 column. A factor X activator was obtained. It had a mol. wt of 12,500 and an isoelectric point of 4.4. It shortened the plasma recalcification time of normal plasma, and plasmas deficient in factors V, VII, VIII, IX, XI and XIII, while it had no effect on plasma deficient in factor X or factor II. It had a serine protease activity and a minimal plasmin activity. PMSF, leupeptin and iodoacetamide exerted a pronounced inhibitory effect on its serine protease activity. Polyantivenin could neutralize the coagulant activity of the activator.

引用

页码：1007 / 1017

页数：11

共 27 条

[1] ALTERATION OF COAGULATION MECHANISMS IN ENVENOMATION BY BOTHROPS-ASPER (VELVET) [J].