DETERMINATION OF POTATO GLYCOALKALOIDS AND THEIR AGLYCONE IN BLOOD-SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - APPLICATION TO PHARMACOKINETIC STUDIES IN HUMANS

被引：60

作者：

HELLENAS, KE

NYMAN, A

SLANINA, P

LOOF, L

GABRIELSSON, J

机构：

[1] SWEDISH UNIV AGR SCI, DEPT PLANT HUSBANDRY, S-75007 UPPSALA, SWEDEN

[2] UNIV HOSP UPPSALA, DEPT INTERNAL MED, S-75185 UPPSALA, SWEDEN

[3] UNIV UPPSALA, DEPT BIOPHARMACEUT & PHARMACOKINET, S-75123 UPPSALA, SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 573卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80476-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of a high-performance liquid chromatography (HPLC) method for the separation and quantification of potato glycoalkaloids and their aglycone solanidine in blood serum is reported. High selectivity was obtained by using solid-phase extraction followed by off-line dual-column HPLC. Injections were made via a sample enrichment column to achieve maximum sensitivity in the assay. The potato alkaloids in the HPLC effluents were detected by ultraviolet absorption at 200 nm. The detection limits were estimated to be 0.3 ng/ml of serum for each of the alkaloids. The method was used to study the pharmacokinetics of potato glycoalkaloids in humans. Alpha-Solanine and alpha-chaconine were detected in all blood serum samples collected from seven volunteers 1-25 h after a meal of potatoes. Solanidine was detected in some samples, but there were no traces of the mono- or diglycosides. The average apparent biological half-lives for alpha-solanine and alpha-chaconine were 11 and 19 h, respectively.

引用

页码：69 / 78

页数：10

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