DNA RELATEDNESS, KARYOTYPING AND GENE PROBING OF CANDIDA-TROPICALIS, CANDIDA-ALBICANS AND ITS SYNONYMS CANDIDA-STELLATOIDEA AND CANDIDA-CLAUSSENII

被引：7

作者：

MAHROUS, M

SAWANT, AD

PRUITT, WR

LOTT, T

MEYER, SA

AHEARN, DG

机构：

[1] Laboratory for Microbial and Biochemical Sciences, Georgia State University, Atlanta, 30303, GA

[2] Division of Bacterial and Mycotic Diseases, Centers for Disease Control, Atlanta, 30333, Ga

来源：

EUROPEAN JOURNAL OF EPIDEMIOLOGY | 1992年 / 8卷 / 03期

关键词：

KARYOTYPING; CANDIDA-ALBICANS; CANDIDA-TROPICALIS; CANDIDA-STELLATOIDEA; CANDIDA-CLAUSSENII;

D O I：

10.1007/BF00158581

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Isolates of Candida albicans with varied phenotypes, including sucrose-negative variants (C. stellatoidea, serotypes A and B) and avirulent germ tube-negative forms (C claussenii) showed significant (> 90%) DNA relatedness to classical C albicans, but insignificant relatedness to C. tropicalis and sucrose-negative C tropicalis. A transverse alternating-field gel electrophoresis procedure (TAFE) showed discrete karyotype patterns among the phenotypic variants of C albicans including the sucrose-negative C stellatoidea. The number of chromosome-sized DNA bands for C. tropicalis (7 bands) were within the range of bands observed for C albicans (5 to 10 bands). The general DNA-migration pattern for C albicans appeared distinct from that of C tropicalis. An aspartyl proteinase (PrA) gene probe from C albicans hybridized with chromosomal DNA from C. albicans, C claussenii and C stellatoidea but not with that from C tropicalis.

引用

页码：444 / 451

页数：8

共 43 条

[1]

Ahearn D.G., Meyer S.A., Mitchell G., Nicholson M.A., Ibrahim A.N., Sucrose-negative variants of Candida tropicalis, J. Clin. Microbiol., 5, pp. 494-651, (1977)

[2]

Ahearn D.G., Schlitzer R.L., Yeast infections, Diagnostic procedures for bacterial, mycotic and parasitic infections, pp. 991-1012, (1981)

[3]

Auger P., Dumas C., Joly J., A study of 666 strains of Candida albicans: Correlation between serotype and susceptibility to 5-Fluorocytosine, J. Infect. Dis., 139, pp. 590-594, (1979)

[4]

Barnett J.A., Payne R.W., Yarrow D., A guide to identifying and classifying yeasts, (1990)

[5]

Brown-Thoen J., Variability in Candida albicans (Robin) Berkhout. I. Studies on morphological and biochemical activities, Hereditas, 60, pp. 355-398, (1968)

[6]

Cole G.T., Lynn K.T., Seshan K.R., Pope L.M., Gastrointestinal and systemic candidosis in immunocompromized mice, Sabouraudia, 27, pp. 363-380, (1989)

[7]

Diddens H.A., Lodder J., Die anaskoporogenen Hefen, II Teil, (1942)

[8]

Gueho E., Meyer S.A., A reevaluation of the genus Malassezia by means of genome comparison, Antonie van Leeuwenhoek, 55, pp. 245-251, (1989)

[9]

Hasenclever H.F., Mitchel W.O., Antigenic studies of Candida. I. Observation of two antigenic groups of Candida albicans, J. Bacteriol., 82, pp. 570-573, (1961)

[10]

Iwaguchi S., Homma M., Tanaka K., Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans, J. Gen. Microbiol., 136, pp. 2433-2442, (1990)

← 1 2 3 4 5 →