ISOLATION AND CHARACTERIZATION OF A DIURETIC PEPTIDE COMMON TO THE HOUSE-FLY AND STABLE FLY

被引：51

作者：

CLOTTENS, FL

HOLMAN, GM

COAST, GM

TOTTY, NF

HAYES, TK

KAY, I

MALLET, AI

WRIGHT, MS

CHUNG, JS

TRUONG, O

BULL, DL

机构：

[1] UNIV LONDON BIRKBECK COLL,DEPT BIOL,LONDON WC1E 7HX,ENGLAND

[2] LUDWIG INST CANC RES,LONDON W1P 8BT,ENGLAND

[3] TEXAS A&M UNIV,INST BIOSCI & TECHNOL,DEPT ENTOMOL,COLLEGE STN,TX 77843

[4] ST THOMAS HOSP,INST DERMATOL,MASS SPECTROMETRY FACIL,LONDON SE1 7EH,ENGLAND

来源：

PEPTIDES | 1994年 / 15卷 / 06期

关键词：

CYCLIC AMP; FLUID SECRETION; MALPIGHIAN TUBULES; MANDUCA SEXTA; MUSCA DOMESTICA; STOMOXYS CALCITRANS; CORTICOTROPIN-RELEASING FACTOR-LIKE PEPTIDES;

D O I：

10.1016/0196-9781(94)90059-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An identical CRF-related diuretic peptide (Musca-DP) was isolated and characterized from whole-body extracts of the house fly, Mucsca domestica, and stable fly, Stomoxys calcitrans. The peptide stimulates cyclic AMP production in Manduca sexta Malpighian tubules and increases the rate of fluid secretion by isolated Musca domestica tubules. The 44-residue peptide, with a mol.wt. of 5180, is amidated, and has the primary structure: NKPSLSIVNPLDVLRQRLLLEIARRQMKENTRQVELNRAILKNV-NH2. Musca-DP has a high percentage of sequence identity with other characterized CRF-related insect diuretic peptides.

引用

页码：971 / 979

页数：9

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