ORGANIC CATION-TRANSPORT IN HUMAN RENAL BRUSH-BORDER MEMBRANE-VESICLES

被引:52
作者
OTT, RJ
HUI, AC
YUAN, G
GIACOMINI, KM
机构
[1] UNIV CALIF SAN FRANCISCO, SCH PHARM, SAN FRANCISCO, CA 94143 USA
[2] UNIV CALIF SAN FRANCISCO, SCH MED, SAN FRANCISCO, CA 94143 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 261卷 / 03期
关键词
N1-METHYLNICOTINAMIDE; TETRAETHYLAMMONIUM; ORGANIC CATION-PROTON ANTIPORTER; SECRETION; SPECIES; LUMINAL MEMBRANE;
D O I
10.1152/ajprenal.1991.261.3.F443
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The renal proximal tubule is responsible for the active elimination of organic cations. Studies in brush-border membrane vesicles (BBMV) suggest that active organic cation transport is mediated by an organic cation-proton antiporter. The goals of this study were to determine whether this transporter is expressed in human kidney and to elucidate its characteristics. Transport of the organic cations N1-[H-3]methylnicotinamide (NMN) and [C-14]tetraethylammonium (TEA) was determined by rapid filtration in BBMV of donor human kidneys. The uptake of NMN and TEA was driven against a concentration gradient by an outwardly directed proton gradient. NMN uptake was inhibited by the organic cations TEA, NMN, quinine, and cimetidine, but was not affected by p-aminohippuric acid, cephalexin, and polyamines. The electroneutral transport of NMN was characterized by a K(m) of 0.44 +/- 0.07 mM and a V(max) of 24.4 +/- 15.2 pmol.mg protein-1.s-1. The rate of proton efflux from BBMV increased in the presence of an inwardly directed TEA gradient. Preloading BBMV with NMN or TEA trans-stimulated the initial rate of uptake of TEA. Therefore the human proximal tubule expresses an organic cation-proton antiporter in the brush-border membrane. The antiporter is similar to that described in other mammalian species but shows some differences in its transport characteristics.
引用
收藏
页码:F443 / F451
页数:9
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