THE MAJOR CYSTEINE PROTEINASE (CRUZIPAIN) FROM TRYPANOSOMA-CRUZI IS ENCODED BY MULTIPLE POLYMORPHIC TANDEMLY ORGANIZED GENES LOCATED ON DIFFERENT CHROMOSOMES

被引:160
作者
CAMPETELLA, O
HENRIKSSON, J
ASLUND, L
FRASCH, ACC
PETTERSSON, U
CAZZULO, JJ
机构
[1] UNIV UPPSALA,CTR BIOMED,DEPT MED GENET,BOX 589,S-75123 UPPSALA,SWEDEN
[2] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,CONICET,INST INVEST BIOQUIM FDN CAMPOMAR,BUENOS AIRES,ARGENTINA
关键词
TRYPANOSOMA-CRUZI; CYSTEINE PROTEINASE; CRUZIPAIN; TANDEMLY REPEATED GENES; CHROMOSOMAL LOCALIZATION;
D O I
10.1016/0166-6851(92)90219-A
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We demonstrate that cruzipain, the major cysteine proteinase of Trypanosoma cruzi epimastigotes, is encoded by a large number of tandemly arranged genes. Restriction enzyme analysis of 20 clones containing complete repeat units of the gene, as well as sequencing of 2 of these clones, and comparison with previously published partial sequences, indicated that the sequence is conserved among the repeat units, although polymorphisms clearly exist. The repeat units contain an intergenic region of 528 bp and coding regions for pre- and pro-enzyme, a central domain and a C-terminal extension. The predicted amino acid sequences of these regions indicated a sequence identity of 30, 60, 70 and 36%, respectively, when the T. cruzi sequence was compared with the sequence of a similar cysteine proteinase from Trypanosoma brucei [14]. Studies by pulsed field gel electrophoresis, complemented with restriction analysis, indicated that the clusters are located on 2-4 different chromosomes in several parasite isolates.
引用
收藏
页码:225 / 234
页数:10
相关论文
共 22 条
[1]   THE C-TERMINAL EXTENSION OF THE MAJOR CYSTEINE PROTEINASE (CRUZIPAIN) FROM TRYPANOSOMA-CRUZI [J].
ASLUND, L ;
HENRIKSSON, J ;
CAMPETELLA, O ;
FRASCH, ACC ;
PETTERSSON, U ;
CAZZULO, JJ .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 45 (02) :345-348
[2]  
CAMPETELLA O, 1990, FEMS MICROBIOL LETT, V67, P145, DOI [10.1111/j.1574-6968.1990.tb13852.x, 10.1016/0378-1097(90)90184-R]
[3]   TRYPANOSOMA-CRUZI RIBOSOMAL-RNA - INTERNAL BREAK IN THE LARGE-MOLECULAR MASS SPECIES AND NUMBER OF GENES [J].
CASTRO, C ;
HERNANDEZ, R ;
CASTANEDA, M .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1981, 2 (3-4) :219-233
[4]   SOME KINETIC-PROPERTIES OF A CYSTEINE PROTEINASE (CRUZIPAIN) FROM TRYPANOSOMA-CRUZI [J].
CAZZULO, JJ ;
FRANKE, MCC ;
MARTINEZ, J ;
DECAZZULO, BMF .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1037 (02) :186-191
[5]   AMINO-ACID AND CARBOHYDRATE-COMPOSITION OF A LYSOSOMAL CYSTEINE PROTEINASE FROM TRYPANOSOMA-CRUZI - ABSENCE OF PHOSPHORYLATED MANNOSE RESIDUES [J].
CAZZULO, JJ ;
HELLMAN, U ;
COUSO, R ;
PARODI, AJA .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 38 (01) :41-48
[6]   END PRODUCTS AND ENZYME LEVELS OF AEROBIC GLUCOSE FERMENTATION IN TRYPANOSOMATIDS [J].
CAZZULO, JJ ;
DECAZZULO, BMF ;
ENGEL, JC ;
CANNATA, JJB .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1985, 16 (03) :329-343
[7]   PROTEIN AND AMINO-ACID CATABOLISM IN TRYPANOSOMA-CRUZI [J].
CAZZULO, JJ .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1984, 79 (03) :309-320
[8]   THE GENOME OF TRYPANOSOMA-CRUZI CONTAINS A CONSTITUTIVELY EXPRESSED, TANDEMLY ARRANGED MULTICOPY GENE HOMOLOGOUS TO A MAJOR HEAT-SHOCK PROTEIN [J].
DRAGON, EA ;
SIAS, SR ;
KATO, EA ;
GABE, JD .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (03) :1271-1275
[9]   AMPLIFICATION AND SEQUENCING OF GENOMIC DNA FRAGMENTS ENCODING CYSTEINE PROTEASES FROM PROTOZOAN PARASITES [J].
EAKIN, AE ;
BOUVIER, J ;
SAKANARI, JA ;
CRAIK, CS ;
MCKERROW, JH .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 39 (01) :1-8
[10]   TRYPANOSOMA-CRUZI EXHIBITS INTER-STRAIN AND INTRA-STRAIN HETEROGENEITY IN MOLECULAR KARYOTYPE AND CHROMOSOMAL GENE LOCATION [J].
ENGMAN, DM ;
REDDY, LV ;
DONELSON, JE ;
KIRCHHOFF, LV .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1987, 22 (2-3) :115-123