PLATELET-ACTIVATING-FACTOR-INDUCED AUGMENTATION OF PRODUCTION OF EOSINOPHIL-LINEAGE CELLS IN HEMATOPOIETIC PRECURSOR CELLS OBTAINED FROM HUMAN UMBILICAL-CORD BLOOD

被引:10
作者
SAITO, H
KOSHIO, T
YANAGIHARA, Y
AKIYAMA, K
SHIDA, T
机构
[1] Clinical Research Center for Rheumato-Allergology, National Hospital of Sagamihara, Sagamihara City, Kanagawa
关键词
PLATELET-ACTIVATING FACTOR; HEMATOPOIETIC PRECURSOR CELLS; UMBILICAL CORD BLOOD; EOSINOPHIL PRODUCTION; INTERLEUKIN-1; INTERLEUKIN-3;
D O I
10.1159/000236586
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Platelet-activating factor (PAF)-induced augmentation of spontaneous differentiation into eosinophil-lineage cells from hematopoietic precursor cells was found among human umbilical cord blood mononuclear cells (CBMCs). This ability of PAF to augment eosinophil production was significantly (p < 0.01) inhibited by the addition of the anti-PAF agent WEB 2086. This enhancing effect of PAF was significantly (p < 0.01) diminished by the presence of antibodies to interleukin (IL)-1 alpha, -beta and IL-3, whereas antibodies to IL-5 or granulocyte-macrophage colony-stimulating factor (GM-CSF) did not diminish it. IL-1 beta, IL-3 or IL-5 gene expression was detected in cellular RNA isolated from both medium- and PAF-stimulated CBMCs using the reverse transcription polymerase chain reaction (RT-PCR) method; however, no expression of GM-CSF was detected. Moreover, 5 times as much IL-3 was detected in the PAF-stimulated CBMC culture supernatant than in that of cells treated with the medium. In the case of IL-1 beta, there was no difference between the 2 cell preparations. On the other hand, no IL-5 or GM-CSF was detected in the supernatant after stimulation with medium or PAF. Depletion of CD2(+), CD16(+) or CD19(+) cells, but not CD14(+) cells, from CBMCs resulted in a marked decrease in PAF-induced augmentation of eosinophil production. These results suggest that PAF induces the augmentation of IL-3 production in CBMCs which in turn enhances differentiation into eosinophil-lineage cells.
引用
收藏
页码:375 / 382
页数:8
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