BRASSINOSTEROIDS - DISTRIBUTION IN PLANTS, BIOASSAYS AND MICROANALYSIS BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引:66
作者
TAKATSUTO, S
机构
[1] Department of Chemistry, Joetsu University of Education, Niigata, 943, Joetsu-shi
关键词
D O I
10.1016/0021-9673(94)85202-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
During the past decade, studies on brassinosteroids (BRs) have greatly widened the knowledge of new steroidal plant hormones. This review summarizes studies on BRs from the viewpoints of distribution in plants, bioassays and a microanalytical method using gas chromatography-mass spectrometry with selected ion monitoring (GC-MS-SIM). A highly sensitive and specific bioassay employed to isolate brassinolide from rape pollen is bean-second internode assay. The rice-lamina inclination test and wheat leaf unrolling test are now widely and routinely used mainly in Japan as highly sensitive and specific bioassays during the purification steps of BRs from the plant sources. When a highly purified fraction containing a very small amount of BRs is obtained, the fraction is derivatized with methaneboronic acid to form a bismethaneboronate of BRs and then analysed by CC-MS-SIM. The rice-lamina inclination test and the GC-MS-SIM microanalytical method have contributed greatly to studies on the identification of many natural BRs and also to their screening and distribution in the plant kingdom. So far about 30 natural BRs have been characterized in a number of higher plants (phanerogams) and also in some lower plants (cryptogams). These data strongly suggest that BRs occur widely in the plant kingdom, as in the case of other known plant hormones, and that BRs play some physiological functions in plant growth and development.
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页码:3 / 15
页数:13
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共 84 条
  • [1] ABE H, 1991, ACS SYM SER, V474, P18
  • [2] ABE H, 1991, ACS SYM SER, V474, P200
  • [3] IDENTIFICATION OF BRASSINOLIDE-LIKE SUBSTANCES IN CHINESE CABBAGE
    ABE, H
    MORISHITA, T
    UCHIYAMA, M
    MARUMO, S
    MUNAKATA, K
    TAKATSUTO, S
    IKEKAWA, N
    [J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (10): : 2609 - 2611
  • [4] A NEW BRASSINOLIDE-RELATED STEROID IN THE LEAVES OF THEA-SINENSIS
    ABE, H
    MORISHITA, T
    UCHIYAMA, M
    TAKATSUTO, S
    IKEKAWA, N
    [J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1984, 48 (08): : 2171 - 2172
  • [5] ENDOGENOUS BRASSINOSTEROIDS OF THE RICE PLANT - CASTASTERONE AND DOLICHOSTERONE
    ABE, H
    NAKAMURA, K
    MORISHITA, T
    UCHIYAMA, M
    TAKATSUTO, S
    IKEKAWA, N
    [J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1984, 48 (04): : 1103 - 1104
  • [6] OCCURRENCE OF 3 NEW BRASSINOSTEROIDS - BRASSINONE, (24S)-24-ETHYLBRASSINONE AND 28-NORBRASSINOLIDE, IN HIGHER-PLANTS
    ABE, H
    MORISHITA, T
    UCHIYAMA, M
    TAKATSUTO, S
    IKEKAWA, N
    IKEDA, M
    SASSA, T
    KITSUWA, T
    MARUMO, S
    [J]. EXPERIENTIA, 1983, 39 (04): : 351 - 353
  • [7] ABE H, 1987, KAGAKU KENKYUHI HOJO, V1, P17
  • [8] BRASSINOSTEROIDS
    ADAM, G
    MARQUARDT, V
    [J]. PHYTOCHEMISTRY, 1986, 25 (08) : 1787 - 1799
  • [9] IDENTIFICATION AND QUANTIFICATION OF BRASSINOLIDE-RELATED STEROIDS IN THE INSECT GALL AND HEALTHY TISSUES OF THE CHESTNUT PLANT
    ARIMA, M
    YOKOTA, T
    TAKAHASHI, N
    [J]. PHYTOCHEMISTRY, 1984, 23 (08) : 1587 - 1591
  • [10] BRASSINOLIDE-RELATED NEW BIOACTIVE STEROIDS FROM DOLICHOS-LABLAB SEED
    BABA, J
    YOKOTA, T
    TAKAHASHI, N
    [J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1983, 47 (03): : 659 - 661