RAPID AND TRANSIENT DECREASE OF N-MYC EXPRESSION IN RETINOIC ACID-INDUCED DIFFERENTIATION OF OTF9 TERATOCARCINOMA STEM-CELLS

被引:54
作者
KATO, K
KANAMORI, A
KONDOH, H
机构
[1] NAGOYA UNIV, SCH SCI, DEPT MOLEC BIOL, NAGOYA 46401, JAPAN
[2] KYOTO UNIV, FAC SCI, DEPT BIOPHYS, KYOTO 606, JAPAN
关键词
D O I
10.1128/MCB.10.2.486
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The level of expression of N-myc in mouse teratocarcinoma stem cells is very high. Previous studies have shown that N-myc expression significantly decreases when the stem cells are subjected to long-term induction for differentiation by retinoic acid (RA). We fond that in a stem cell line, OTF9, a steep yet transient decrease of N-myc expression takes place much earlier, immediately after induction by RA. To examine whether this decrease is responsible for differentiation, we constructed a gene, miwNmyc, to express N-myc cDNA constitutively and transformed OTF9 cells with this gene construct. Transformants under the constitutive expression of mivNmyc differentiated normally, as judged by morphological changes and by modulation of c-myc, Hox1.1, and laminin B1 expression. Therefore, transient decrease of N-myc expression may be the consequence of RA-induced differentiation, even though it occurs very early in the process. Alternatively, in addition to N-myc decrease, there may be redundant mechanisms which lead to OTF9 differentiation after induction by RA, so that suppression of N-myc decrease is bypassed by at least one other mechanism.
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页码:486 / 491
页数:6
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