CHROMOSOME LOCALIZATION OF HUMAN ARH GENES, A RAS-RELATED GENE FAMILY

The human ARH genes (previously called RHO) share several properties with the ras gene family. Three members of the ARH family, the H6, H9, and H12 genes, have been localized to human chromosomes 2, 5, and 3, respectively. Analysis of DNAs from a rodent-human somatic cell hybrid panel demonstrates linkage of H6 to chromosome region 2p12→2pter and H9 to region 5q33→5qter. In situ chromosome hybridization also showed that the primary site for H9 is in the 5q31→-qter region. The H12 gene was some-what difficult to localize using rodent-human hybrids because the probe detects a family of rodent genes as homologous to the human probe as is the human cognate gene. However, chromosome in situ hybridization revealed grains clustered in region 3p14→3p22 with a significant peak in band 3p21. We conclude that H6 is in 2p12→pter, H9 in 5q31→5qter, and H12 in 3p21. © 1990.