TRANSFECTED F3 F11 NEURONAL CELL-SURFACE PROTEIN MEDIATES INTERCELLULAR-ADHESION AND PROMOTES NEURITE OUTGROWTH

被引：158

作者：

GENNARINI, G

DURBEC, P

BONED, A

ROUGON, G

GORIDIS, C

机构：

[1] UNIV BARI,FAC MED & CHIRURG,IST FISIOL UMANA,I-70124 BARI,ITALY

[2] CNRS,URA 179,F-13288 MARSEILLE 9,FRANCE

来源：

NEURON | 1991年 / 6卷 / 04期

关键词：

D O I：

10.1016/0896-6273(91)90062-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The mouse neuronal F3 glycoprotein and its chicken homolog F11 belong to a subclass of proteins of the immunoglobulin superfamily with preferential localization on axons and neurites. We have transfected F3 cDNA into CHO cells. Biochemical analysis establishes that the cDNA we have cloned codes for a 130 kd phosphatidylinositol-anchored polypeptide. F3-expressing transfectants exhibited enhanced self-adhesive properties, aggregating with faster kinetics and forming larger aggregates than F3-negative control cells. When used as a culture substrate for sensory neurons, F3-transfected cells showed a markedly enhanced ability to promote neurite outgrowth compared with nontransfected cells. The results support the idea that F3/F11 and other closely similar proteins function as cell adhesion molecules that play a role in axonal growth and guidance.

引用

页码：595 / 606

页数：12

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