DIFFERENTIAL SCANNING CALORIMETRIC STUDIES OF ESCHERICHIA-COLI ASPARTATE-TRANSCARBAMYLASE .3. THE DENATURATIONAL THERMODYNAMICS OF THE HOLOENZYME WITH SINGLE-SITE MUTATIONS IN THE CATALYTIC CHAIN

Aspartate transcarbamylase (EC 2.1.3.2) from E. coli is a multimeric enzyme consisting of two catalytic subunits and three regulatory subunits whose activity is regulated by subunit interactions. Differential scanning calorimetric (DSC) scans of the wild-type enzyme consist of two peaks, each comprised of at least two components, corresponding to denaturation of the catalytic and regulatory subunits within the intact holoenzyme (Vickers, J. Biol. Chem. 253 (1978) 8493; Edge, Biochemistry 27 (1988) 8081). We have examined the effects of nine single-site mutations in the catalytic chains Three of the mutations (Asp-100-Gly, Glu-86-Gln, and Arg-269-Gly) are at sites at, the C1 : C2 interface between c chains within the catalytic subunit. These mutations disrupt salt linkages present in both the T and R states of the molecule (Honzatko, J.L Mol. Biol. 160 (1982) 219; Krause et al., J. Mol. Biol. 193 1987) 527). The remainder (Lys-164-Ile, Tyr-165-Phe, Glu-239-Gln, Glu-239-Ala, Tyr-240-Phe and Asp-271-Ser) are at the C1 : C4 interface between catalytic subunits and are involved in interactions which stabilize either the T or R state. DSC scans of all of the mutants except Asp-100-Gly and Arg-269-Gly consisted of two peaks. At intermediate concentrations, Asp-100-Gly and Arg-269-Gly had only a single peak near the Tm of the regulatory subunit transition in the holoenzyme, although their denaturational profiles were more complex at high and low protein concentrations. The catalytic subunits of Glu-86-Gln, Lys-164-Ile and Asp-271-Ser appear to be significantly destabilized relative to wild-type protein while Tyr-165-Phe and Tyr-240-Phe appear to be stabilized. Values of ΔΔ°cr, the difference between the subunit interaction energy of wild-type and mutant proteins, evaluated as suggested by Brandts (Biochemistry 28 (1989) 8588) range from - 3.7 kcal mol-1 for Glu-86-Gln to 2.4 kcal mol-1 for Tyr-165-Phe. © 1990.